Tang Xianglian,Liu Denghui,Li Ming,et al.Effect of mir-29b in the proliferation,invasion and migration of neuroblastoma cells[J].Journal of Clinical Pediatric Surgery,,19():711-716.[doi:10.3969/j.issn.1671-6353.2020.08.010]
Effect of mir-29b in the proliferation,invasion and migration of neuroblastoma cells
- Keywords:
- Neuroblastoma; Neoplasm Invasiveness; Neoplasm Metastasis
- CLC:
- R739.4;R730.2
- Abstract:
- Objective To detect the expression of miR-29b in adrenal gland and neuroblastoma and examine the effect of miR-29b on the proliferation, invasion and migration of neuroblastoma cells. Methods The expression of miR-29b was detected by real-time quantitative polymerase chain reaction (PCR) in neuroblastoma tissues and paracancerous adrenal tissues. And miR-29b related nucleotides (mimics/inhibitor/NC) were transiently transferred into neuroblastoma SK-N-SH cells. The growth inhibition of miR-29b on SK-N-SH was determined bv CCK-8 assay, cell migration and invasion ability was evaluated by Transwell invasion and Scratch test and the expressions of epithelial transformation related proteins (E-cadherin & vimentin) were detected by Western blot. Results miR-29b became down-regulated in neuroblastoma tissues compared with that in paracancerous adrenal tissues (P<0.05). After transfection with miR-29-related nucleotides (mimics/inhibitor/NC), miR-29b mimics exhibited the effect of inhibiting cell proliferation (P<0.05); Transwell tests showed that the migrated cells in miR-29b mimics group (36.33±8.08) were less than that in miR-29b inhibitor (112.30±11.68) and NC group (112.30±11.68); Scratch test showed that the healing rate (51.67±4.50) of mir-29b was significantly lower than that of mir-29b inhibitor group (87.67±3.79) and NC group (85.00±3.00). And migration and invasion ability of K-N-SH cells decreased significantly (P<0.05); Western blot showed that the expression of E-cadherin increased in miR-29b mimics group and the expression of Vimentin declined while the opposite result appeared in miRNA-29b inhibitor group (P<0.05). Conclusion miR-29b is down-regulated in neuroblastoma tissues. And an overexpression of miR-29b can inhibit cell proliferation, invasion and migration of neuroblastoma SK-N-SH cells. It may be related with an inhibition of epithelial mesenchymal transition (EMT).
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Memo
收稿日期:2019-09-25。
基金项目:湖南省自然科学基金面上项目（编号：2018JJ2210）
通讯作者:李勇,Email:liyongpuwaike@163.com