Comparison of two different methods to establish animal model of hydronephrosis due to incomplete ureteral obstruction.[J].Journal of Clinical Pediatric Surgery,,10():107-110.
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 Comparison of two different methods to establish animal model of hydronephrosis due to incomplete ureteral obstruction.

Journal of Clinical Pediatric Surgery[ISSN:1671-6353/CN:43-1380/R] volume: 第10卷 Number: 2011 02 Page: 107-110 Column: Date of publication: 2011-04-28
Keywords:
 ; Ureteral Obstruction; Uronephrosis; Modela; Animal
Document code:
A
Abstract:
 【Abstract】ObjectiveTo establish and compare two different methods of hydronephrosis due to ureteropelvic junction obstruction in rabbit.MethodsTwentyfour New Zealand rabbits （10weekold，Male） were randomly assigned to two groups: group A （12 rabbits） were embedded the right ureter of ureteropelvic junction under the psoas major muscle and group B（12 rabbits） were emboliaed the right ureter of ureteropelvic junction into a plastic cannula. All rabbits were performed both on SPECT and CT perfusion preoperative and postoperative respectively, and parameters including GFR, thickness of renal cortical, diameter of pelvis, BV, BF, MTT and PS were assessed and the statistical significance of differences was determined, in terms of BUN、CR、ultrasound result、pathologic diagnoses pathology result and statistical analyses were performed simultaneously on the GFR of SPECT and CT perfusion parameters.ResultsThe success rate of animal model was 83.3% in group A and 91.7% in group B, respectively. All models presented chronic hydronephrosis by pathologically confirmed. BUN and CR of right kidney both group A and group B were increased after operation, Perfusion parameters including BV、BF、PS and GFR of both group A and group B were decreased but rate of decline of group A was more significant than group B （P<0.05）. Both of group showed dilation of renal calyx and pelvis, as well as there was statistically significant difference between the two sets（P＜0.05）.ConclusionsBoth two method provide highly feasibility for hydronephrosis in rabbit model , but we would recommend method of plastic cannula is the best and first the choice.

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