- Keywords:
- ; Spermatic Cord Torsion; Reperfusion Injury; Rats
- Document code:
- A
- Abstract:
- 【Abstract】ObjectiveTo study the injury of the contralateral testicular after after unilateral testicular torsion in prepubertal rats, and the protective effects of Sildenafil against the injury of contralateral testicular spermatogenic function after unilateral testicular torsion. Methods72 prepubertal SpragueDawley rats were divided randomly into three groups(n=24):shamoperation group、 saline group and Sidenafil group. All the rats except shamoperation group underwent torsion/detorsion operation that rotated left testicle 720 °clockwise and kept in torsion for 4 hours. All the rats except shamoperation group received corresponding drug orally before anesthesia. In all groups, The right (contralateral) testicle were removed from each groups(n=8) 4 hours、24 hours and 2 weeks respectively after the operation for histopathological analysis as well as the test of malondialdehyde (MDA) and NO/NOS levels. Results4 hours after the operation: the histopathological change of testes, MDA and NOS levels were not statistically different among three groups. However, the NO level has a obviously increase in Sildenafil group than other two groups(P<0.05 ). 24 hours after the operation: in the saline (placebo)group, histopathological change of testes were much worse than shamoperation group and Sidenafil group . However, compared with the shamoperation group, the test levels of MDA and NO/NOS were statistically higher in saline group and Sidenafil group(P<0.05).in those two groups, saline group has the higher levels than Sidenafil group(P<0.05). 24 hours after the operation: the histopathological analysis show there are most serious injury of contralateral testes of the saline group.Compared with the shamoperation group, the test levels of MDA and NO/NOS were statistically higher in saline group and Sidenafil group(P<0.05).in those two groups, saline group has the higher levels than Sidenafil group(P<0.05).ConclusionsThe contralateral testicular has no significant injury after unilateral testicular torsion/detorsion in prepubertal rats for 4hous later. It turns injury at 24 hours after the torsion/detorsion operation, and sustained until 2weeks later. Sidenafil has a protective effect on contralateral testicle after unilateral testicular torsiondetorsion injury, though the protective effect of Sidenafil seems to increase the NO level in in focal organizations, dilate the blood vessel and antagonize the blood vessel constriction of sympathetic nerves.
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