Zhou Hui,Li Suolin,Shi Baojun,et al.Hepatic expression of PDGF-BB in infants with biliary atresia and its correlation with epithelial mesenchymal transition[J].Journal of Clinical Pediatric Surgery,,22():238-243.[doi:10.3760/cma.j.cn101785-202012064-007]
Hepatic expression of PDGF-BB in infants with biliary atresia and its correlation with epithelial mesenchymal transition
- Keywords:
- Biliary Atresia; Platelet-Derived Growth Factor; Epithelial-Mesenchymal Transition; Protein Biosynthesis
- Abstract:
- Objective To explore the hepatic expression of platelet-derived growth factor-BB (PDGF-BB),Snail 1 and epithelial-mesenchymal transition (EMT) in children of biliary atresia (BA) and examine the relationship among three parameters.Methods From January 2018 to December 2019,there were BA (n=25),choledochal cysts (CCC,n=15) and liver tumors (NL,n=10).After collecting biopsy tissues,immunohistochemistry and real-time fluorescent quantitative polymerase chain reaction (PCR) were employed for detecting the expression of PDGF-BB,Snail 1,E-cadherin and N-cadherin and analyzing their correlations.Results mRNA and protein expressions of PDGF-BB,Snail 1 and N-cadherin were significantly higher in liver of BA group than those of CCC/NL group (P<0.05); mRNA and protein expressions of E-cadherin were significantly lower than that of CCC/NL group (P<0.05).PDGF-BB was correlated positively with Snail 1 mRNA and protein expressions (r=0.772,P<0.05; r=0.780,P<0.05); PDGF-BB was correlated positively with N-cadherin mRNA and protein expressions (r=0.835,P<0.05; r=0.702,P<0.05); PDGF-BB and E-cadherin mRNA and protein expressions were correlated negatively (r=-0.756,P<0.05; r=-0.696,P<0.05); Snail 1 and N-cadherin mRNA and protein expression were correlated positively (r=0.723,P<0.05; r=0.702,P<0.05); Snail 1 was correlated negatively with E-cadherin mRNA and protein expressions (r=-0.720,P<0.05; r=-0.636,P<0.05).Conclusion In BA children,PDGF-BB may promote EMT through enhancing the transcription activity of transcription factor Snail 1.Thus it may provide new theoretical rationales for diagnosing and treating BA children.
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Memo
收稿日期:2020-12-30。
基金项目:河北省政府资助临床医学优秀人才培养计划项目(2019008)
通讯作者:李索林,Email:lisuolin@263.net