Sun Chongrui,Zhang Mingjie,Xu Zhuoming..Structure of gut microbiota in children with single ventricle physiology.[J].Journal of Clinical Pediatric Surgery,,17():620-625.
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Structure of gut microbiota in children with single ventricle physiology.

Journal of Clinical Pediatric Surgery[ISSN:1671-6353/CN:43-1380/R] volume: 第17卷 Number: 2018 08 Page: 620-625 Column: 实验研究 Date of publication: 2018-08-28
Author(s):
Sun Chongrui; Zhang Mingjie; Xu Zhuoming.
Department of Cardiothoracic Surgery,Shanghai Children’s Medical Center,Shanghai Jiao Tong University School of Medicine,Shanghai 200127,China.
Keywords:
Single Ventricle;  Heart Diseasea/CN;  Gut Microbiota;  Highthroughput sequencing
Document code:
A
Abstract:
ObjectiveTotal DNA of stool samples was extracted from children with single and biventricle physiology by Illumina highthroughput sequencing technology and the differences of intestinal microbiota between two groups were compared.MethodsTwenty cases of singleventricle children (11 males,9 females,age of 3~10 years) undergoing total cavopulmonary connection were selected as singleventricle group while 20 biventricle children of matching gender and age (±1 year) as biventricle group.Total bacterial DNA was extracted from stool samples of two groups and 16S rRNA gene was sequenced by highthroughput sequencing.Then the structural data of intestinal microflora were obtained.ResultsAt the level of operational taxonomic units,no significant intergroup difference existed in richness index (Ace:190.88 vs.201.28 and Cho:188.27 vs.201.90),diversity index (Shannon:3.140 vs.3.090 and Simpson:0.109 vs.0.110) and coverage index (coverage:0.999 vs.0.999) of gut microbiota (P>0.05).The predominant 4 phyla of intestinal microbiota were Firmicutes,Bacteroidetes,Proteobacteria and Actinobacteria.In addition,Cyanobacteria had significant intergroup difference (P<0.05).A total of 224 different genera of bacteria were obtained in two groups,among which Faecalibacterium (9.55% vs.14.91%),Veillonella (3.08% vs.0.50%),Lachnoclostridium (1.02% vs.2.12%),Roseburia (0.62% vs.2.51%),Bilophila (0.25% vs.0.05%) and Actinomyces (0.312% vs.0.008%) had significant intergroup difference (P<0.05).ConclusionNo significant difference exists in diversity index of gut microbiota between single and doubleventricle groups.However,the structure of intestinal microbiota has changed in some specific bacteria.
Last Update: 2018-08-21