LIU De hong,SHEN Li ping,CAI Lei,et al.Establishment of testicle leydig cells specific Cre transgenic mice[J].Journal of Clinical Pediatric Surgery,,12():201-204.[doi:10.3969/j.issn.1671—6353.2013.03.011]
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Establishment of testicle leydig cells specific Cre transgenic mice

Journal of Clinical Pediatric Surgery[ISSN:1671-6353/CN:43-1380/R] volume: 第12卷 Number: 2013 03 Page: 201-204 Column: 论著 Date of publication: 2013-06-28
Author(s):
LIU Dehong1; 3;  SHEN Liping1;  CAI Lei3;  et al.
1，Ruijin Hospital Shanghai Jiaotong University， Medicine of School， Shanghai 200025，China；2，Biomodel Organism Center of Shanghai， Shanghai 200127，China；3，Beijing children’s Hospital，Capital Medical University， Beijing 100045，China.
Keywords:
Ｔｅｓｔｉｓ;  Ｓｔｒｏｍａｉ　Ｃｅｌｌｓ; 　 Ａｎｉｍａｉ　Ｅｘｐｅｒｉｍｅｎｔａｔｉｏｎ;  Ｇｅｎｅ　Ｅｘｐｒｅｓｓｉｏｎ
DOI:
10.3969/j.issn.1671—6353.2013.03.011
Abstract:
Objetive Amhr2 protein is the specific protein of testicle tissue. We generated a new transgenic line expressing Cre recombinase under the control of an Amhr2 gene promoter (Amhr2Cre) to investigate sexual development.MethodsMicroinjection was employed to introduce the 7.1 kb transgenic fragment into oocytes，and progenies were obtained. PCR detected expression of Amhr2Cre transgene within testicle tissue containing leydig cells. Transgenic line was crossed with ROSA26 report line， the activity of Cre recombinase was revealed by LacZ staining in ROSA26; Amhr2Cre double transgenic mice.ResultsPCR products revealed that the Amhr2 gene was deleted by Cre mediated recombination in the leydig cells. LacZ staining of the Amhr2Cre and ROSA26 double transgenic mice showed that Cre recombinase activity was detected in leydig cells.ConclusionAll these data indicated that the Cre recombinase has been expressed in the testicle tissues of the Amhr2Cre transgenic mice．The mice generated could serve as a useful tool for generating testicle specific geneknockout mice.

References:

1Xu Q， Lin HY， Yeh SD， et al. Infertility with defective spermatogenesis and steroidogenes is in male mice lacking androgen receptor in Leydig cells［J］.Endocrine，2007，32 (1):96—106.
2程萱，陈红星，杨晓，等．提高制备转基因小鼠效率的研究［J］．中国实验动物学报，2001，9(3):160．
3周江，程萱，吕娅歆，等．胰腺组织表达Cre重组酶转基因小鼠的建立及鉴定［J］．生物工程学报，2002，18(3):28．
4Hughes IA， Martin H， Jskelinen J. Genetic mechanisms of fetal male undermasculinization: a background to the role of endocrine disruptors［J］. Environ Res，2006，100(1):44—49.
5Hughes IA. Disorders of sex development: a new definition and classification［J］.Best Pract Res Clin Endocrinol Metab，2008，22(1):119—134.
6李远伟， 赵晓昆， 高智勇，等. ATF3在单纯性尿道下裂中的表达及意义［J］. 临床小儿外科杂志，2008，7(4):29—32.
7杨超，魏光辉. 尿道下裂的病因学研究进展［J］. 临床小儿外科杂志，2008，7(5):57—58.
8Wang Y， Jia Y， Franken P， et al. Loss of APC function in mesenchymal cells surrounding the Müllerian duct leads to myometrial defects in adult mice［J］. Mol Cell Endocrinol，2011，341(1—2):48—54.
9Daikoku T， Jackson L， Besnard V， et al. Cellspecific conditional deletion of Pten in the uterus results in differential phenotypes［J］. Gynecol Oncol，2011，122(2):424—429.
10Harman RM， Cowan RG， Ren Y， et al. Reduced signaling through the hedgehog pathway in the uterine stroma causes deferred implantation and embryonic loss［J］. Reproduction，2011，141(5): 665—674.
11Kyrnlahti A， Euler R， Bielinska M， et al. GATA4 regulates Sertoli cell function and fertility in adult male mice［J］.Mol Cell Endocrinol， 2011， 333(1):85—95.
12LaguMN， Detmar J， Paquet M， et al. Decidual PTEN expression is required for trophoblast invasion in the mouse［J］. Am J Physiol Endocrinol Metab，2010，299(6): 936—946.

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基金项目：国家自然科学基金项目(编号：30700830)

Last Update: 2013-06-30