Deng Xiaobin,Peng Liang,Zhao Zhenzhen,et al.Clinical application of circulating tumor cells in the diagnosis and prognosis of neuroblastoma[J].Journal of Clinical Pediatric Surgery,,22():741-746.[doi:10.3760/cma.j.cn101785-202301021-008]
Clinical application of circulating tumor cells in the diagnosis and prognosis of neuroblastoma
- Keywords:
- Circulating Tumor Cells; Neuroblastoma; Diagnosis; Prognosis
- Abstract:
- Objective To elucidate the relationship between circulating tumor cells (CTCs) and clinicopathological characteristics of neuroblastoma (NB) and explore the clinical application value of CTCs detection in the diagnosis and treatment of NB.Methods A total of 87 children with pathologically diagnosed NB from January 2021 to September 2022 were selected as study group.Clinical data, laboratory tests and CTCs test results were recorded.At the same time, 20 healthy children were selected as negative controls.A total of 6 mL of venous anticoagulant blood from each subject was collected and CTCs were enriched by specific antibody nanofluidic chip immunoassay with CytoSorter? CTCs sorting system and identified by anti-CSV Ab and anti-GD2 Ab.SPSS26.0 software and Graphpad Prism 9 were employed for data processing and graphing.Results CTCs were detected in 67.8% NB children and in 5% healthy controls.Significant differences existed between two groups (P<0.01).CTCs positive rate was correlated with age, tumor size, International Neuroblastoma Risk Group (INRG) stage, risk stratification, bone/bone marrow metastasis, survival status and 24 h VMA level, yet not with gender, tumor type, Shimada classification, lymph node metastasis or Mycn amplification.CTCs number was higher in children aged above 18 months, uFH classification, tumor size >5 cm and with bone/bone marrow metastases.The number of CTCs was higher in more advanced stage and higher risk stratification children as well as in deceased individuals.The longest follow-up time in this group was 2.5 years, the overall survival rate of this study was 53.9% and the survival rate of CTCs-positive children was significantly lower than that of CTCs-negative counterparts (46.8% vs.87.1%, P=0.002).Taking CTCs as a diagnostic criteria of NB, the efficacy was higher than that of urinary VMA.Conclusion CTCs have a high detection rate in NB children and its positive rate is correlated with unfavorable clinical prognostic factors.And CTCs may be used as an indicator for evaluating the prognosis of NB.
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Memo
收稿日期:2023-01-29。
基金项目:重庆市科卫联合医学科研项目（2021MSXM073）
通讯作者:杨超,Email:400843@hospital.cqmu.edu.cn