XING Li li,WU Ye ming,ZHANG Chi..Ursolic acid effects proliferation、apoptosis and MYCN expression of neuroblastoma cell[J].Journal of Clinical Pediatric Surgery,,12():196-200.[doi:10.3969/j.issn.1671—6353.2013.03.010]
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Ursolic acid effects proliferation、apoptosis and MYCN expression of neuroblastoma cell

Journal of Clinical Pediatric Surgery[ISSN:1671-6353/CN:43-1380/R] volume: 第12卷 Number: 2013 03 Page: 196-200 Column: 论著 Date of publication: 2013-06-28
Author(s):
XING Lili;  WU Yeming;  ZHANG Chi.
Department of Pediatric Surgery, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.
Keywords:
Neuroblastoma;  Ursolic acid;  Cell proliferation;  Cell apoptosis;  MYCN
DOI:
10.3969/j.issn.1671—6353.2013.03.010
Abstract:
Objetive This study was designed to investigate the effects of ursolic acid in proliferation、apoptosis and MYCN gene mRNA expression of SHSY5Y cell line cultured in vitro, in order to investigate the effect of ursolic acid (UA) in neuroblastoma(NB).MethodNeuroblastoma SHSY5Y cells were treated by 4 uM/L、8 uM/L、12 uM/L ursolic acid.WST1 was used to measure the levels of the proliferation of SHSY5Ycells cultured with different concentrations of ursolic acid. While SHSY5Ycells apoptosis was measured by flow cytometry. MYCN gene mRNA expression in SHSY5Yceils was measured using real time PCR.ResultsThe proliferation of SHSY5Ycells was obviously inhibited by ursolic acid in a doseand timedependent manner. The apoptosis of SHSY5Ycells was obviously promoted by ursolic acid in a dosedependent manner. The MYCN gene mRNA expression in SHSY5Ycells was obviously inhibited by ursolic acid in a doseependent manner. Ursolic acid of 12uM/L treatment for 72 hrs produced a best effect with an inhibitory rate of SHSY5Y cell proliferation of 98.3 % and inhibitory rate of MYCN gene mRNA expression of 52.37%. ConclusionsUrsolic acid may inhibit the growth of neuroblastoma cells, promote neuroblastoma cells apoptosis and downregulate MYCN mRNA expression.

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Last Update: 2013-06-30