WANG Dong,HU Bo,ZHAN Jiang-hua.An Experimental Study on the Therapeutic Effect of Transplanted Neural Stem cells in Aganglionic Megacolon Rats Model[J].Journal of Clinical Pediatric Surgery,,12():124-127.[doi:10.3969/j.issn.1671— 6353.2013.02.014]
An Experimental Study on the Therapeutic Effect of Transplanted Neural Stem cells in Aganglionic Megacolon Rats Model
- Keywords:
- Aganglionic; Neural stem cells; transplantation; Rat; Functional improvement
- Abstract:
- Objetive To elucidate the potential and the biological significance of grafting of central nervous system-derived NSCs(CNS-NSCs)as a therapeutic strategy for neuronal replacement in disorders of the enteric nervous system(ENS)such as aganglionic megacolon. Methods To induce chemical ablation of the enteric plexus in rat to produce an aperistaltic narrow segment simulating to Hirschsprung’s disease, the cationic surfactant BAC was applied to a 2 cm serosal surface of the rectum. CNE — NSCs were transplanted into the denervated segment of rectum. After 2、4 and 8 weeks grafted cells were visualized in rectum tissue sections by fluorescent staining for neuronal and astrocytic markers. Results 60 rats arrived after chemical induce animal model. We separated into 2 groups(n=30 for each group). We can see the alive NSCs in the enteric wall(22/30)after being transplanted into the denervated gut after 2 weeks injection. 4 weeks later, NSCs could survive and could differentiate into neurons and glial cells in vivo. At the 8-week assessment, some transplanted NSCs exhibited immunophenotypes of neuronal and glail cell. Barium enema show us there are enteric function improvement after NSCs transplantation. Conclusions Grafted CNS — NSCs can survive anddifferentiation, And restore the denervated rectum of rat in vivo nerve function to a certain extent, which indicates that NSCs provide a promising cellular replacement candidate for treatment of aganglionosis and some other disorder of the enteric nervous system.
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