CHEN Ren wei,ZHU Xin yuan,ZHOU Wen wu,et al.Protective effects of ulinastatin on cellular immune function in infants underwent cardiac operations with cardiopulmonary bypass.[J].Journal of Clinical Pediatric Surgery,,10():0.
Protective effects of ulinastatin on cellular immune function in infants underwent cardiac operations with cardiopulmonary bypass.
- Keywords:
- Extracorporeal Circulation; Lymphocytes/IM; Infant
- Abstract:
- ObjectiveTo observe the change of T lymphocyte immunity functions after cardiopulmonary bypass (CPB) in infants, and to evaluate the protective effects of ulinastatin on cellular immune function. MethodsFrom Jan. 2010 to Jun. 2010, there were 50 infants with ventricular septal defects, whom divided randomly into the Ulinastatin group or Control, enrolled into this prospective trial. And infants from the ulinastatin group were treated with 1000 U/kg which was added into the CPB priming fluid during the correct operations. Blood samples were taken at different time points for the analysis of T lymphocyte and its subtype by flow cytometry.Results24 hours after the correct operations, and compared with the control group, infants from the ulinastatin group had higher levels of CD4+, CD3+ and CD4+/CD8+, as well as a lower level of CD8+. And 7days late, the levels of CD4+, CD3+ and CD4+/CD8+ in the ulinastatin group were still higher than those in the control group.ConclusionsAdministration of ulinastatin in the CPB priming fluid can attenuate the inhibition of T lymphocyte immunity functions caused by CPB in infants.
References:
1Misoph M,Babin Ebell J,Schwender S,et al.Response of the cellular immune system to cardiopulmonary bypass in vivo \[J\].Thorac Cardiovasc Surg,1997,45(5):217—223.
2Markew itz A,Faist E, L ang S, et al.An imbalance in Thelpercell subsets alters immune response after cardiac surgery\[J\].Eur J Cardiothorac Surg,1996,10(1):61—67.
3Ma T,Kang C, Shao H,et al.Protective effects of ulinastatin on proliferation and cytokine release of splenocytes from rats with severe acute pancreatitis \[J\].Eur Surg Res, 2006,38(5):445—450.
4郭巍,邹承伟,王安彪,等.西咪替丁对体外循环手术患者免疫功能的保护作用\[J\].中国胸心血管外科临床杂志, 2007, 14(2): 97—100.
5雷宾峰,陈铭伍,冼磊,等.乌司他丁对心脏不停跳心内直视术机体免疫功能的影响\[J\].广西医科大学学报, 2008, 25(3):381—383.
Memo
作者单位：湖南省儿童医院心胸外科（长沙市，410007），本课题受湖南省自然科学基金资助（项目号06TJ41078)