Yan Peihong,Zheng Yi,Chen Houping,et al.Mechanism of plasminogen activator inhibitor1 promoting liver fibrosis in biliary atresia.[J].Journal of Clinical Pediatric Surgery,,17():790-794.
Mechanism of plasminogen activator inhibitor1 promoting liver fibrosis in biliary atresia.
Journal of Clinical Pediatric Surgery[ISSN:1671-6353/CN:43-1380/R]
volume:
第17卷
Number:
2018 10
Page:
790-794
Column:
实验研究
Date of publication:
2018-10-25
- Keywords:
- Biliary atresia; Liver fibrosis; PAI1; TGFβ1; Smad3; PSmad3
- Document code:
- A
- Abstract:
- ObjectiveTo explore the expression of plasminogen activator inhibitor1 (PAI1) of transforming growth factor beta 1 (TGFβ1) profibrotic signaling pathway in liver of biliary atresia (BA) and provide theoretical rationales for elucidating the mechanism of liver fibrosis in BA.MethodsA total of 55 children diagnosed and treated at Tianjin Childrens Hospital from January 2010 to July 2016 were divided into three groups,including those dying of normal nonhepatobiliary diseases (control group,n=5),congenital biliary dilatation (biliary dilatation group,n=25) and Kasai operation with biliary atresia (Kasai group,n=25).And another 25 patients underwent liver transplantation at First Central Municipal Hospital of Tianjin from January 2013 to January 2016 (transplantation group,n=25).The tissue structure and the degree of liver fibrosis were observed by hematoxylin & eosin (HE) staining.And immunohistochemical staining was used for detecting the expressions of Smad3,PSmad3 and PAI1. ResultsHE staining showed that there was no fibrosis in liver in control group.There were a few inflammatory cells and fibroblast proliferation in biliary dilatation group and without bile duct hyperplasia and obvious fibrosis.In Kasai group,fibroblasts and collagen fibers proliferated extensively and bridging fibrosis occurred.In transplantation group,massive fibrous tissue hyperplasia,pseudolobular formation and cirrhosis could be found.Immunohistochemical staining showed that the expressions of Smad3,PSmad3 and PAI1 in Kasai group were significantly higher than three other groups (P<0.000).The differences were statistically significantly.Smad3 content was higher than PSmad3 content while PSmad3 was equivalent to PAI1.The PAI1 content (0.03±0.01) in hepatic lobules of Kasai group was higher than that in portal area (0.002±0.001) (t=13.76,P<0.000).The differences was statistically significant.ConclusionPAI1 is mostly expressed in Kasai group and decreases in transplantation group.It implies that PAI1 has profibrotic effect on early stage of BA.As the degree of liver fibrosis advances,profibrotic effect gradually subdues.
Last Update:
2018-10-24