Li Mingyong,Wang Xing,Shi Qinlin,et al.Exprssion of Smad7,Smad3 and Smad2 in the hypospadiac penis of fetal rats induced by DEHP.[J].Journal of Clinical Pediatric Surgery,,17():136-140.
Exprssion of Smad7,Smad3 and Smad2 in the hypospadiac penis of fetal rats induced by DEHP.
Journal of Clinical Pediatric Surgery[ISSN:1671-6353/CN:43-1380/R]
volume:
第17卷
Number:
2018 02
Page:
136-140
Column:
实验研究
Date of publication:
2018-02-28
- Keywords:
- Hypospadias; Diethylhexyl Phthalate; Smad7 Protein; Smad3 Protein; Smad2 Protein; Rats
- Document code:
- A
- Abstract:
- ObjectiveTo investigate the expression of Smads (7,3 and 2) in the hypospadias penis of fetal rats induced by maternal exposure to di(2ethylhexyl) phthalate (DEHP),in order to explore the etiology of hypospadias.MethodsPregnant rats were randomly divided into experiment group and control group,20 rats for each group.Each rat in the experiment group was treated with DEHP dissolved in 1.5ml soybean oil by savaging,while control group were treated with 1.5ml soybean oil,once a day from the 12th gestation days (GD12) to GD19.Fetal rats were obtained on the GD20 by caesarean section,and were investigated for the mRNA and protein expression levels of Smad7,Smad3 and Smad2 by using realtime quantitative PCR and immunohistochemistry assay respectively.ResultsThe expression of Smad7,Smad3 and Smad2 mRNA in hypospadias rats foreskin tissue increased 97.0%,22.8% and 59.9% times compared with normal control groups,respectively,and the differences were significant (all P<0.05).Moreover,the protein levels of Smad7 and phosphorylated Smad 2/3 in foreskin from children with hypospadias showed an incremental trend.ConclusionOur results preliminary indicate that Smads(7,3 and 2) overexpression might be associated with the DEHPinduced congenital hypospadias.
Last Update: