Sun Qiang,Ye Jiajun,Zhou Ying.Epidemiological survey and influencing factors of developmental dysplasia of the hip in Tibetan children of Sichuan and Tibet[J].Journal of Clinical Pediatric Surgery,,22():1050-1054.[doi:10.3760/cma.j.cn101785-202204079-009]
Epidemiological survey and influencing factors of developmental dysplasia of the hip in Tibetan children of Sichuan and Tibet
- Keywords:
- Developmental Dysplasia of the Hip; Joint Dislocations; Epidemiologic Studies; Tibetan Nationality; Child
- Abstract:
- Objective The construction of animal models of neonatal necrotizing enterocolitis (NEC) is still not uniform,and animal modeling approaches that are more relevant to the actual clinical situation of NEC childern should be clarified.Methods Fifty-four neonatal mice were randomly divided into five groups: control group (Ctrl),hypoxia + artificial feeding group (HF),hypoxia + artificial feeding + cold stimulation group (Cold),hypoxia + artificial feeding + lipopolysaccharide group (LPS),and hypoxia + artificial feeding + intestinal bacteria in NEC group (Bac).After the animal models of NEC were established,the intestinal pathology,NEC-related intestinal epithelial barrier proteins (β-catenin and Occludin),intestinal epithelial cell death (CC3,RIPK1 and PARP1) and pro-inflammatory cytokines (IL-6,TNF-α and MCP1) were evaluated.Results Nadler score ≥2 according to intestinal histology was considered as NEC-like intestinal injury.In this study,the intestinal histopathology of the three NEC-modeled groups met the criteria for NEC-like intestinal injury,except for the Ctrl and HF groups.Compared with the NEC modeling groups HF (30%),Cold (83.3%) and LPS (81.8%),the Bac group had the highest modeling success rate (100%),and the mental status,bloating and diarrhea,and mobility of the mice in the Bac group during the modeling period were more consistent with clinical NEC.Meanwhile,the expression of intestinal barrier proteins β-catenin and Occludin was decreased in the Bac group mice,and the difference was statistically significant compared with the Ctrl group (P<0.05).the expression of intestinal epithelial cell death marker molecules RIPK1 and PARP1 was upregulated in the LPS and Bac groups,and the expression levels of inflammatory factors IL-6,TNF-α and MCP1 were increased compared with the Ctrl group,with statistically significant differences (P<0.05).Conclusions In this study,we successfully compared four animal models of NEC and identified an animal modeling method,namely "hypoxia+artificial feeding+intestinal bacteria in NEC",that is more relevant to the actual situation of clinical NEC children.This modeling method has a high success rate,and the intestinal histopathological damage,intestinal barrier protein expression and systemic inflammatory response are more similar to the characteristics of clinical NEC children.
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Memo
收稿日期:2022-4-26。
基金项目:四川省医学会医学科研课题(S19065); 四川省中医药管理局四川省名中医周英工作室活态传承建设项目(川中医药办发[2022]16号)
通讯作者:孙强,Email:2357401669@qq.com.