Li Yanyun,Liu Xuelai,Cui Zhao,et al.Histological observation of TLR4/MyD88 signaling pathway in septum arisen from type Ⅰ intestinal atresia[J].Journal of Clinical Pediatric Surgery,,22():180-184.[doi:10.3760/cma.j.cn101785-202202018-015]
Histological observation of TLR4/MyD88 signaling pathway in septum arisen from type Ⅰ intestinal atresia
- Keywords:
- Intestinal Atresia; Etiology; Intestinal Atresia; Pathology; Septum; Immunology; Toll-like Receptor Signaling Pathway
- Abstract:
- Objective To observe the expressions of TRIF,TRAF3,IFN-β and IFN-λ1 in septum derived from type I intestinal atresia and examine their correlations.Methods Immunohistochemistry was utilized for observing and comparing the expressions of important molecules in TRIF pathway in mucosa of normal intestinal wall and type I intestinal atresia diaphragm.A total of 16 tissue samples were collected from Department of Pediatric Medicine,General Hospital of PLA(n=1),Capital Institute of Pediatrics(n=1),Second Hospital,Hebei Medical University(n=6),Harbin Children’s Hospital(n=5)and Changchun Children’s Hospital(n=3).All samples were intraoperatively confirmed as type I intestinal atresia diaphragm tissue(diaphragm group).Normal intestinal wall tissues collected during intestinal resection and anastomosis in the same child were selected as control group.Totally 16 septa in operated neonates at post-birth 1~3 days were collected,including 13 septa(male 5,female 8)derived from jejunum and 3 septa(male 1,female 2)from ileum.Normal intestinal wall tissues were collected as control during surgery in the same neonate.All fresh tissues were processed for serial tissue sectioning and hematoxylin-eosin(HE)& immunohistochemical stains of TRIF,TRAF3,IFN-β and IFN-λ1 molecules in each group for histological observations.Four visual fields under immunohistochemical stain were targeted randomly for semi-quantitative calculation of average optical density(OD)by Image pro plus6.0 software.Statistical methods of t test and double blinding were employed for analyzing the average optical density of immunohistochemistry.Results Immunohistochemical stain indicated that the expressions of TRIF,TRAF3,IFN-β and IFN-λ1 in septum tissue were significant compared with those in normal neonatal intestinal wall.Semi-quantitative calculation indicated marked elevations in septum tissue in contrast to those in normal neonatal intestinal wall(fraction of diaphragm tissue was 0.6 316±0.0 268,0.5 066±0.0 378,0.6 091±0.0 270,0.3 841±0.0 610;normal intestinal wall 0.4 333±0.0 409,0.3 758±0.0 365,0.4 108±0.0 263,0.2 108±0.0 573,p value point ratio 0.0042,0.0 360,0.0 057,0.0 137).Conclusion TLR4/TRIF signaling pathway is involved in formation and development of septum tissue in type I intestinal atresia during fetal period.
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Memo
收稿日期:2021-8-31。
基金项目:北京市自然科学基金(7222015)
通讯作者:刘雪来,Email:liuxuelai_steven@163.com