Gao Runnan,Zhu Haitao,Shen Chun.Research advances in models of necrotizing enterocolitis in vivo and in vitro[J].Journal of Clinical Pediatric Surgery,,21():331-335.[doi:10.3760/cma.j.cn101785-202009024-007]
Research advances in models of necrotizing enterocolitis in vivo and in vitro
- Keywords:
- Enterocolitis; Necrotizing; Models of Necrotizing Enterocolitis; Organoids; Epithelial Cells; Models; Animal; Intestines
- Abstract:
- Necrotizing enterocolitis (NEC) has been a leading cause of gastrointestinal emergencies in premature infants.Over the last 50 years,a large variety of experimental models have been developed for examining the pathophysiology of NEC and testing the effectiveness of new therapeutic strategies.Current etiological studies of NEC have employed intestinal epithelial cells and animal models.However,cell models cannot mimic the biological characteristics of intestinal tissues in children while animal models reflect human diseases and other related characteristics.With the latest discovery of intestinal stem cell marker Lgr5+,a novel in vitro intestinal model is gradually emerging.This review focused upon the relevant experimental models and discussed the main advantages and disadvantages of each model so as to help researchers better select proper NEC experimental models.
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Memo
收稿日期:2020-09-14;改回日期:。
基金项目:国家自然科学基金面上项目(81873849);上海市自然科学基金面上项目(22ZR1408600);国家儿童医学中心青年临床科学家项目(EK112520180306)
通讯作者:沈淳,Email:chunshen@fudan.edu.cn