Pan Lijia,Wu Yeming,Wang Yi,et al.Application of near infrared-indocyanine green imaging system for judging ischemic intestinal segment in a canine model of acute intestinal ischemia[J].Journal of Clinical Pediatric Surgery,,20():925-929.[doi:10.12260/lcxewkzz.2021.10.006]
Application of near infrared-indocyanine green imaging system for judging ischemic intestinal segment in a canine model of acute intestinal ischemia
- Keywords:
- Fluorescent Dyes; Indocyanine Green; Diagnostic Imaging; Acute Intestinal Iischemia; Reperfusion Injury; Animal Model
- CLC:
- R917.76;R574;R445.9
- Abstract:
- Objective To apply near infrared-indocyanine green (NIR-ICG) imaging system can assess intestinal blood supply through tracing blood flow with indocyanine green (ICG) in a canine model of acute intestinal blood supply disorder.Methods An acute ischemic intestinal model was established by non-destructively clamping a section of canine jejunal/ileal mesentery and blocking all relevant branches of jejunal and ileal blood vessels.After an intravenous injection of ICG, the changes of intestinal fluorescent intensity were tracked at different timepoints and the system’s software was utilized for real-time semi-quantitative analysis.Results ICG 1 mg/kg was injected after modeling.After 1 min, ischemic intestine remained invisible while adjoining normal intestine was distinctly visualized.Fluorescent intensity of normal intestine was defined as 100% and ischemic intestine as 0%.After 3 min, fluorescent intensity of ischemic intestine was still 0%.Then reperfusion injury model was established with an injection of ICG fluorescent intensity was defined as 100% at 0 min.After 120 min, fluorescent intensity of injured intestinal segment declined to 20.3% while adjoining normal intestinal segment dropped to 0%.Conclusion Capable of distinguishing ischemic intestinal segments quickly, NIR-ICG imaging system provides an objective and accurate new tool for judging intestinal ischemia during surgery.
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Memo
收稿日期:2021-02-22。
基金项目:国家自然科学基金（编号：81874234）；苏州市临床医学专家团队引进项目（编号：SZYJTD201706）；上海市市级医院新兴前沿技术联合攻关项目（编号：SHDC12018123）；上海市生物医药领域科技支撑项目（编号：18401931800）；上海市转化医学协同创新中心项目（编号：TM201809）
通讯作者:吴晔明,Email:wuyeming@xinhuamed.com.cn