Hu He,Jiang Fei,Ji Guangchun,et al.Effect of light repair on spinal cord tether with neurogenic bladder in rats[J].Journal of Clinical Pediatric Surgery,,19():648-652.[doi:10.3969/j.issn.1671-6353.2020.07.017]
Effect of light repair on spinal cord tether with neurogenic bladder in rats
- Keywords:
- Neural Tube Defects; Urinary Bladder; Neurogenic; Light Gene; Nerve Growth Factor; Child
- CLC:
- R726.9;R694+.5;R628
- Abstract:
- Objective To evaluate the efficacy of photoreceptor gene therapy for spinal cord tethering with neurogenic bladder in a juvenile rat model preliminarily.Methods Forty-six female young rats were randomly divided into three groups of healthy control (15 rats),tethered cord with light gene (15 rats) and tethered cord without light gene (16 rats).The juvenile rat model of spinal cord tethered with neurogenic bladder was established.The transfection of adenovirus vector Ad-EGFP-ChR2 was observed by immunofluorescent staining.After 2-week specific blue light irradiation,bladder urodynamics,enzyme-linked immunosorbent assay (ELISA) and Western blot were performed for detecting nerve growth factor (NGF) in urine and bladder tissues.Results .As compared with tethered cord without light gene group,bladder detrusor leakage point pressure and residual urine volume decreased,maximum volume and compliance increased,NGF content in urine dropped,NGF/Cr value declined,the expression of NGF protein in bladder tissue decreased in tethered cord with light gene group. The difference in the above indicators was statistically significant.As compared with healthy control group,no significant difference existed in the above indices of tethered cord without light gene group (P>0.05).Conclusion The results of animal experiments show that photorecepter gene technology may restore neurogenic bladder functions induced by spinal cord thrombosis.It provides experimental therapeutic rationales for children with spinal cord tether with neurogenic bladder dysfunction.
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Memo
收稿日期:2019-05-12。
基金项目:辽宁省科学技术基金（编号：20170540216）
通讯作者:蒋飞,Email:jf2299@163.com