Cheng Jiwen,Zhao Pu,Yang Weili,et al.Let-7b modulates the proliferation of hepatoblastoma cells by targeting SALL4[J].Journal of Clinical Pediatric Surgery,,19():404-409.[doi:10.3969/j.issn.1671-6353.2020.05.006]
Let-7b modulates the proliferation of hepatoblastoma cells by targeting SALL4
- Keywords:
- let-7b; Liver Neoplasms; SALL4; Cell Proliferation
- CLC:
- R726;R735.7
- Abstract:
- Objective To explore the role and mechanism of let-7b and SALL4 in the malignant behaviors of hepatoblastoma (HB) cells. Methods Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was employed for defecting the expression of let-7b in normal liver cells (HL-7702),HB cell lines,HB tissues and adjacent normal tissues.Immunohistochemistry was applied for measuring the expression levels of SALL4 in HB tissues and paracancerous tissue and analyzing the correlation between the expression levels of SALL4 in HB tissues.Up-regulating or inhibiting the let-7b expression level in HB cells was used for examining the effects of let-7b on the biological function of HB cells by methyl thiazolyl tetrazolium (MTT) assay,cell cycle and apoptosis.The targeting relationship between let-7b and SALL4 was assessed through dual-luciferase reporter system,qRT-PCR,Western blot and in vitro immunohistochemical staining. Results let-7b became markedly down-regulated in HB tissues and HB cells (e.g.HepG2 & HuH6) while SALL4 protein was frequently up-regulated in HB tissues.The expression of let-7b was correlated with PRETEXT stage (P=0.002).A significant negative correlation existed between let-7b and SALL4 expression level in HB tissue samples (r=-0.761,P<0.001).Through the detection of proliferative activity,cell cycle and cell apoptosis,let-7b could regulate the proliferating capability of HepG2.The dual-luciferase reporter assays revealed that SALL4 was a novel target of let-7b.Conclusion Serving as a tumor suppressor gene,let-7b suppresses the HepG2 cells growth by targeting SALL4.
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Memo
收稿日期:2019-09-01。
基金项目:陕西省创新人才推进计划-青年科技新星项目（编号：2018KJXX-050）；西安交通大学中央高校基本科研业务项目（编号：XTT2018126，YT（ZD）201704）
通讯作者:李鹏,Email:sienafiat@hotmail.com