Wang Tianyi,Zhang Weiping,Han Wenwen,et al.Expression and significance of platelet-derived growth factor receptor alpha-positive cells in stenotic tissue of congenital ureteropelvic junction obstruction[J].Journal of Clinical Pediatric Surgery,,19():213-217.[doi:10.3969/j.issn.1671-6353.2020.03.005]
Expression and significance of platelet-derived growth factor receptor alpha-positive cells in stenotic tissue of congenital ureteropelvic junction obstruction
- CLC:
- R726.9;R692.7
- Abstract:
- Objective To observe the expression of platelet-derived growth factor receptor α-positive (PDGFRα+) cells in congenital ureteropelvic junction obstruction (UPJO) for exploring their role in the pathogenesis of congenital hydronephrosis in children.Methods UPJO group was composed of specimens of ureteropelvic junction (UPJ) harvested during pyeloplasty(n=30) while control group involved the specimens of normal ureter (nephrectomy for tumor; n=10).The immunofluorescence of specimens was examined by light microscopy and computerized image analysis for the expression and distribution of PDGFRα+ cells in each group.In addition,small conductance calcium-activated potassium channel 3 (SK3) and closely associated cells consisting of smooth muscle cells (SMCs),interstitial cells of Cajal (ICCs) and nerve fibers were examined.Results Located in close proximity to SMCs,ICCs and nerve fibers,PDGFRα+ cells expressed SK3 channels capable of mediating purinergic inhibitory neurotransmission in SMCs.The expression of SK3 channels decreased obviously in UPJ obstruction in PDGFRα+ cells as compared with controls.Conclusion There is a down-regulation of SK3 channels in UPJO in PDGFRα+ cells.And SK3 channels may be involved in the pathogenesis of UPJO by perturbing normal peristalsis of human UPJ.
References:
1 Jackson L,Woodward M,Coward RJ.The molecular biology of pelvi-ureteric junction obstruction[J].Pediatr Nephrol,2018,33:553-571.DOI:10.1007/s00467-017-3629-0.
2 张潍平,杨洋,汪添益.关于肾盂输尿管连接部梗阻病因和治疗的认识与争议[J].临床小儿外科杂志,2018,17(6):401-404.DOI:10.3969/j.issn.1671-6353.2018.06.001. Zhang WP,Yang Y,Wang TY.Recent advances in the etiology and treatment of ureteropelvic junction obstruction[J].J Clin Ped Sur,2018,17(6):401-404.DOI:10.3969/j.issn.1671-6353.2018.06.001.
3 Hunziker M,O’Donnell AM,Puri P.Platelet-derived gr-owth factor receptor alpha-positive cells:a new cell type in the human ureteropelvic junction[J].Pediatr Res,2017,82(6):1080-1087.DOI:10.1038/pr.2017.193.
4 Solari V,Piotrowska AP,Puri P.Altered Expression of Interstitial Cells of Cajal in Congenital Ureteropelvic Junction Obstruction[J].J Urol,2003,170(6):2420-2422.DOI:10.1097/01.ju.0000097401.03293.f0.
5 Senol C.Changes in Cajal cell density in ureteropelvic junction obstruction in children[J].J Pediatr Urol,2016,12(2):89.e1-e5.DOI:10.1016/j.jpurol.2015.08.010.
6 Iino S,Horiguchi K,Horiguchi S,et al.c-Kit-negative fibroblast-like cells express platelet-derived growth factor receptor alpha in the murine gastrointestinal musculature[J].Histochem Cell Biol,2009,131(6):691-702.DOI:10.1007/s00418-009-0580-6.
7 Monaghan KP,Johnston L,McCloskey KD.Identification of PDGFR alpha positive populations of interstitial cells in human and guinea pig bladders[J].J Urol,2012,188(2):639-647.DOI:10.1016/j.juro.2012.03.117.
8 Zhang PL,Peters CA,Rosen S.Ureteropelvic junction obstruction:morphological and clinical studies[J].Pediatr Nephrol,2000,14(8-9):820-826.DOI:10.1007/s004679900240.
9 Di Benedetto A,Arena S,Nicotina PA,et al.Pacemakers in the upper urinary tract[J].Neurourol Urodyn,2013,32(4):349-353.DOI:10.1002/nau.22310.
10 Lu C,Huang X,Lu HL,et al.Different distributions of interstitial cells of Cajal and platelet-derived growth factor receptor-α positive cells in colonic smooth muscle cell/interstitial cell of Cajal/platelet-derived growth factor receptor-α positive cell syncytium in mice[J].World J Gastroenterol,2018,24(44):4989-5004.DOI:10.3748/wjg.v24.i44.4989.
11 Koh SD,Ward SM,Sanders KM.Ionic conductances regulating the excitability of colonic smooth muscles[J].Neurogastroenterol Motil,2012,24(8):705-718.DOI:10.1111/j.1365-2982.2012.01956.x.
12 Blair PJ,Rhee PL,Sanders KM,et al.The significance of interstitial cells in neurogastroenterology[J].J Neurogastroenterol Motil,2014,20(3):294-317.DOI:10.5056/jnm14060.
13 Lee H,Koh BH,Peri LE,et al.Purinergic inhibitory regulation of murine detrusor muscles mediated by PDGFRα+ interstitial cells[J].J Physiol,2014,592(6):1283-1293.DOI:10.1113/jphysiol.2013.267989.
14 Klemm MF,Lang RJ.Distribution Of Ca2+-Activated K+Channel (SK2 and SK3) Immunoreactivity In Intestinal Smooth Muscles Of The Guinea-Pig[J].Clin Exp Pharmacol Physiol,2002,29(1-2):18-25.DOI:10.1046/j.1440-1681.2002.03601.x.
15 Yeoh JW,Corrias A,Buist ML.A mechanistic model of a PDGFRα+ cell[J].J Theor Biol,2016,408:127-136.DOI:10.1016/j.jtbi.2016.08.004.
Memo
收稿日期:2019-10-20。
基金项目:北京市自然科学基金（编号：7172073）；北京市医管局儿科协同发展专项子课题（编号：XTZD20180303）
通讯作者:张潍平,Email:zhangwpp@163.com