Zhang Ming,Peng Qiang,Liu Ming.Expression of NF-kappa B in propranolol treatment for infantile hemangioma in nude mice model[J].Journal of Clinical Pediatric Surgery,,18():655-659.[doi:10.3969/j.issn.1671-6353.2019.08.008]
Expression of NF-kappa B in propranolol treatment for infantile hemangioma in nude mice model
- Keywords:
- Hemangioma; Nuclear transcription factor; Propranolol; Treatment
- CLC:
- R732.2;R730.51
- Abstract:
- Objective To explore the expression and mechanism of nuclear transcription factor-kappa B (NF-κB) in propranolol treatment of infantile hemangioma nude murine model. Methods Proliferative infantile hemangioma tissue was transplanted into nude mice for establishing the model of hemangioma.Fifty surviving mice were randomly divided into two groups of propranolol and saline solution (control).At Days 7,14,21 and 28 post-intervention,five nude mice from each group were randomly sacrificed by cervical dislocation.The samples of transplanted tumor were tested for NF-kappa Bp65,IκB-αand Bcl-2 by immunohistochemistry.And the expression of NF-kappa Bp65 mRNA in samples was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results After intervention,the transplanted tumors in propranolol group shrank,became lighter and hardened.In saline group,transplanted tumors enlarged initially at Days 7 and 14 and there were numerous hemangioma endothelial cells.Then at Days 21 and 28,tumor tissue shrank,became lighter and hardened.At each timepoint,NF-κBp65 in propranolol group was lower than that in saline group (P<0.05); At various timepoints post-intervention,the expression of NF-kappa Bp65 was lower in ropranolol group than that in normal saline group.The average optical density of NF-kappa Bp65 was 0.12±0.07 pre-intervention and 0.02±0.11 at Day 28 post-intervention.The value of NF-kappa 65 mRNA in propranolol group was 0.981±0.479 pre-intervention and 0.267±0.115 at Day 28 post-intervention.IκB-αwas higher in propranolol group than that in saline group (P<0.05); Bcl-2 was lower in propranolol group than that in saline group (P<0.05).At the same time,a positive linear correlation existed between NF-kappa Bp65 and Bcl-2 in propranolol group (r=0.901,P<0.01).During each phase,NF-kappaBp65 mRNA was significantly lower in propranolol group than that in saline group (P<0.05). Conclusion Propranolol may up-regulate the expression of kappa b-alpha and down-regulate the expressions of NF-kappa Bp65 and bcl-2 in hemangioma tissues of nude mice.The mechanism by which propranolol promotes the regress of hemangioma in nude murine model may be related to its effect of suppressing the anti-apoptosis mediated by NF-kappa B.
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Memo
收稿日期:2019-07-08。
通讯作者:彭强,Email:pengqiangpq@sina.com