Wang Bingjie,Zhou Junmei,Li Lingling,et al.Muscle components of gubernaculum testis from high/low cryptorchidic children[J].Journal of Clinical Pediatric Surgery,,18():523-526,528.[doi:10.3969/j.issn.1671-6353.2019.06.017]
Muscle components of gubernaculum testis from high/low cryptorchidic children
- Keywords:
- Cryptorchidism/ED; Muscles/UL
- CLC:
- R697+.22;R726.9;R322.7+4
- Abstract:
- Objective To explore the relationship between muscle components in gubernaculum testis and different positions of undescended testis in cryptorchidic children. Methods Gubernaculum testis was harvested from cryptorchidic children during orchidopexy.And the samples were divided into high and low groups according to the position of undescended testis under anesthesia.If the position of undescended testis was higher than that of inner ring,the sample was classified as high group (n=11).Contrarily,the position between inner ring and outer ring was designated as low group (n=62).Gubernaculum tissues were observed and compared by different stains of histological sections.Results No muscle or muscle-like structure was found in high group while striated muscle structures with scattered arrangement in 43/62 patients of low group.After hematoxylin-eosin staining,gubernaculum testis of high group was characterized largely by dense connective tissue,numerous fibroblasts and few small blood vessels without striated muscle tissue structure.However,tissues of low group showed mixed components.Striated muscle was composed of loosely arranged fibroblasts,few blood vessels and some fibers.After Masson’s staining,tissue structure was dense with collagen fibers and few small blood vessels in high group.Contrarily,loose collagen fiber components,small blood vessels and striated muscle structure were present in low group.After striated muscle-specific immunofluorescent MY-32 staining,no positive signal was detected in high group while obvious striated muscle structure existed in low group. Conclusion The muscle composition in gubernaculum of cryptorchidic children is different between high and low position of undescended testis.Striated muscle exists in human gubernaculum testis of low group but not in high group.
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Memo
收稿日期:2019-03-20。
基金项目:国家自然科学基金（编号：81870459/81470911）；上海交通大学"医工交叉"基金（编号：YG2016MS32）
通讯作者:陈方,Email:doctorchenfang@126.com