Huang Enfu,Chen Jiao,Cao Xu,et al.Preliminary study of MicroRNA differential expression in hypospadias[J].Journal of Clinical Pediatric Surgery,,18():335-340.[doi:10.3969/j.issn.1671-6353.2019.04.018]
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Preliminary study of MicroRNA differential expression in hypospadias

Journal of Clinical Pediatric Surgery[ISSN:1671-6353/CN:43-1380/R] volume: 第18卷 Number: 2019 04 Page: 335-340 Column: 实验研究 Date of publication: 2019-04-28
Author(s):
Huang Enfu1;  Chen Jiao1;  Cao Xu1;  Feng Xiao2;  Zhang Ya2;  Zhou Yun1
1. Department of Urology, Affiliated Children’s Hospital, Soochow University, Suzhou 215003, China;
2. Institute of Pediatrics, Affiliated Children’s Hospital, Soochow University, Suzhou 215003, China
Keywords:
Hypospadias/ET; MicoRNAs; HCG; RT-PCR
CLC:
R726.9;R393
DOI:
10.3969/j.issn.1671-6353.2019.04.018
Abstract:
Objective To detect the differential expression of miRNAs in foreskin tissue related to hypospadiac deformation in etiology,explore the possible role for preoperative human chorionic gonadotropin (HCG) treatment for facilitating surgical operation and reduce the complications.Methods Foreskin tissue was collected for total RNA extraction from 9 cases of hypospadiac children including 3 cases with preoperative HCG treatment and 3 children with phimosis as control.Chip was used for screening the differential expression of miRNA and the results were verified by real-time polymerase chain reaction (RT-PCR).Results Results of miRNA chips:Compared to children with phimosis,the number of up-regulated miRNA was 19 while down-regulated miRNA was 15 in foreskin tissue of hypospadiacs.As compared to hypospadiacs with preoperative HCG treatment,the number of up-regulated miRNA was 19 while down-regulated miRNA was 25 in foreskin tissue of those without preoperative treatment.Results of RT-PCR:Among 10 differentially expressed miRNAs,half of them were verified,i.e.let-7b-3p,miR-145-5p,miR-376a-3p,miR-377-3p and miR-1-3p.And let-7b-3p,miR-145-5p,miR-376a-3p,miR-377-3p were down-regulated in hypospadias group and up-regulated in phimosis and hypospadias with preoperative HCG treatment.Yet miR-1-3p was up-regulated in hypospadias with preoperative HCG treatment as compared to hypospadias group.Conclusion This study has established the miRNA expression profiles of hypospadias for the first time.Differentially expressed miRNAs are screened among children with hypospadias,those with phimosis and those with hypospadias with preoperative HCG treatment.Future studies of differentially expressed miRNAs may provide new etiological clues for hypospadias.

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Memo

收稿日期:2017-06-13。
基金项目:江苏省临床医学科技专项（编号：BL2012051）；苏州市民生科技项目（编号：SS201754）
通讯作者:周云,Email:cloudyzy@189.cn

Last Update: 1900-01-01