Zhu Guanghui,Mei Haibo,Liu Kun,et al.Expressions of OPG and RANKL in tibia bone and periosteum of patients with congenital tibial pseudarthrosis of the tibia in children[J].Journal of Clinical Pediatric Surgery,,18():331-334.[doi:10.3969/j.issn.1671-6353.2019.04.017]
Expressions of OPG and RANKL in tibia bone and periosteum of patients with congenital tibial pseudarthrosis of the tibia in children
- Keywords:
- Congenital Pseudarthrosis of The Tibia; Osteoprotegerin/CH; Receptor Activator of Nuclear Factor-kappa B/CH; Immunohistochemistry/MT
- CLC:
- R726.8;R682.1+9;R392.11
- Abstract:
- Objective To explore the expressions of osteoprotegerin (OPG) and nuclear factor kappa B receptor activator ligand (RANKL) in tibia bone and periosteum of patients with congenital tibial pseudarthrosis of the tibia (CPT) and tibial fracture in children and analyze its possible significance.Methods From January 2016 to February 2018,17 cases of tibia bone and periosteum of CPT and 10 cases of tibia bone and periosteum of tibial fracture were collected.Strep Avidin-Biotin Complex method was employed for measuring the expressions of OPG and RANKL in bone and periosteum of two groups.Results The expression of OPG in tibial periosteum of CPT patients was lower than tibial fracture counterparts[(0.08±0.03) vs.(0.11±0.02),t=2.805,P<0.01].And the expression of RANKL in tibial periosteum of CPT patients was higher than tibial fracture counterparts[(0.24±0.07) vs.(0.15±0.04),t=3.707,P<0.01].RANKL/OPG ratio was higher in tibial periosteum of CPT than that in tibial fracture counterparts[(2.37±0.29) vs.(1.09±0.16),t=12.792,P<0.01].The expression of OPG was lower in tibial bone of CPT patients than that in tibial fracture counterparts[(0.17±0.09) vs.(0.26±0.05,t=2.895,P<0.01].And the expression of RANKL was higher in tibial bone of CPT patients than that in tibial fracture counterparts[(0.68±0.13) vs.(0.33±0.09),t=7.494,P<0.01].RANKL/OPG ratio was higher in tibial bone of CPT patients than that in tibial fracture counterparts[(5.12±0.60) vs.(1.12±0.167,t=25.784,P<0.05].All differences were statistically significant.Conclusion RANKL/OPG imbalance may be one of the causes of nonunion and bone resorption in CPT children.
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Memo
收稿日期:2018-10-20。
基金项目:湖南省卫计委课题（编号：B2016032）；湖南省自然科学基金青年基金（编号：2017JJ3140）；湖南省儿童医院"青年英才"培养计划
通讯作者:梅海波,Email:meihaibo@sina.com