Sun Rong,Wu Zhouguang,Ye Yongqin,et al.Preliminary study on the expression level and clinical significance of GPC1 in liver tissues of children with biliary atresia[J].Journal of Clinical Pediatric Surgery,,18():211-215.[doi:10.3969/j.issn.1671-6353.2019.03.010]
Preliminary study on the expression level and clinical significance of GPC1 in liver tissues of children with biliary atresia
- Keywords:
- GPC1; Biliary Atresia; Liver/Anatomy &; Histology; Gene Expression Regulation
- CLC:
- R729;R657.4+4;R393
- Abstract:
- Objective To explore the clinical significance of GPC1 in the pathogenesis of children with biliary atresia (BA).Methods A total of 35 hospitalized BA children at Children’s Hospital of Shenzhen from July 2016 to February 2018 were recruited into observation group.And another 42 hospitalized children with congenital choledochal dilatation were selected as control group.The distribution of GPC1 protein in observation group was detected by immunohistochemistry and the expression levels of GPC1 and protein expression in liver samples of two groups were detected by quantitative polymerase chain reaction (qPCR) and Western blot.Results GPC1 was distributed predominantly at the top of cholangiocarcinoma cells.The expression of GPC1 mRNA in observation and control groups (0.65±0.05 vs. 2.54±0.12,P<0.05) and protein expression (1.31±0.14 vs. 2.83±0.25,P<0.05) showed statistical differences.Conclusion The expression of GPC1 may be closely correlated with the pathogenesis of BA.
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Memo
收稿日期:2018-09-13。
基金项目:国家自然科学基金（编号：81770512）
通讯作者:王斌,Email:szwb1967@126.com