Li Y,Xiao YL,Li M,et al.Expression and significance of Apelin/APJ in pediatric neuroblastoma.[J].Journal of Clinical Pediatric Surgery,,18():154-158.
Expression and significance of Apelin/APJ in pediatric neuroblastoma.
Journal of Clinical Pediatric Surgery[ISSN:1671-6353/CN:43-1380/R]
volume:
第18卷
Number:
2019 02
Page:
154-158
Column:
实验研究
Date of publication:
2019-02-25
- Keywords:
- Neuroblastoma; Apelin/APJ System; Tumor Progression; Child
- CLC:
- R729 R735.7 R392-33
- Document code:
- A
- Abstract:
- ObjectiveTo detect the expression and significance of putative receptor protein related to angiotensin receptor ATl (Apelin/APJ) in pediatric neuroblastoma (NB) and explore the relationship between Apelin/APJ system and its occurrence and development.MethodsA total of 10 cases of NB tissues and adjacent adrenal tissues were collected.The expressions of Apelin and APJ protein in tumor and adjacent tissues were detected by immunohistochemistry.Realtime polymerase chain reaction (PCR) and Western blot were employed for detecting the expression levels of Apelin/APJ mRNA and protein.Comparison of plasma levels of Apelin36 was conducted by ELISA in NB children versus healthy controls.ResultsImmunohistochemistry indicated that the expressions of Apelin/APJ mRNA and protein were strongly positive in NB tissues.As compared with adjacent adrenal tissues in control group,the expression levels of Apelin/APJ mRNA and protein were significantly higher in NB tissues than those in control group (P<0.05).And the plasma level of Apelin36 in NB children was significantly higher than that in healthy controls (P<0.05).ConclusionApelin/APJ system is highly expressed in NB tissues.It suggests that the above system is closely correlated with the occurrence and development of pediatric NB.
Last Update:
2019-02-26