Chen Licai,Li Pengfei,Huang Yimin,et al. Targeting therapy of antiEpCAM monoclonal antibody in hepatoblastoma animal model.[J].Journal of Clinical Pediatric Surgery,,18():63-68.
Click Copy

 Targeting therapy of antiEpCAM monoclonal antibody in hepatoblastoma animal model.

Journal of Clinical Pediatric Surgery[ISSN:1671-6353/CN:43-1380/R] volume: 第18卷 Number: 2019 01 Page: 63-68 Column: 论著 Date of publication: 2019-01-20
Author(s):
 Chen Licai; Li Pengfei; Huang Yimin; Liu Jiangbin; Lv Zhibao
 Department of General Surgery,Shanghai Children’s Hospital,Shanghai Jiao Tong University,Shanghai 200062,China.
Keywords:
 ; Hepatoblastoma;  EpCAM;  Antibodies;  Monoclonal;  Targeting Therapy;  Models;  Animal
Document code:
A
Abstract:
 ObjectiveTo explore the efficacy of antiEpCAM (epithelial cell adhesion molecule) monoclonal antibody for hepatoblastoma (HB) in a nude mice model.MethodsHB cell line of HuH6 was cultured and the expression of EpCAM in HuH6 examined.After HuH6 cell was inoculated subcutaneously,all mice were randomly divided into treatment and control groups.In treatment group,antiEpCAM monoclonal antibody was injected into mice by tail vein (30 ug/kg qd daily) for 3 weeks.In control group,the same volume of saline was applied.The expression of EpCAM from implanted tumor mass was detected by immunohistochemistry.Weight and volume of implanted tumor were measured and then tumor inhibition rate was calculated 1 week later.ResultsHuH6 cell was successfully subcultured and the expression of EpCAM was positive on cell membrane.After 6week cell implantation,mass was confirmed as HB by histopathological examination.Compared with control group,antiEpCAM monoclonal antibody could significantly inhibit tumor growth in control group.Tumor weight and volume were smaller in treatment group than those in control group ［tumor weight:(35.6±2.97) vs.（45.8±4.59) mg (P<0.05); tumor size:(1.12±0.09) vs.(1.37±0.77) cm3 (P<0.05)］.The positive rate of EpCAM expression was (28.3±5.4)% in tumor tissue of experimental group and (39.7±4.4)% in tumor tissue of control group.The positive rate of EpCAM expression was lower in tumor tissue of experimental group than that of control group.And the difference was statistically significant (P<0.05). ConclusionAntiEpCAM monoclonal antibody can inhibit the growth of HB tumor cells in a murine model.And it serves as a potential monoclonal targeting therapy for pediatric HB.
Last Update: 2019-01-22