Hu Shuqi,Zhao Xiaoxia,Qin Qi,et al.The bowel morphological and functional variations in short bowel syndrome of murine:an invitro study.[J].Journal of Clinical Pediatric Surgery,,17():383-389.
The bowel morphological and functional variations in short bowel syndrome of murine:an invitro study.
Journal of Clinical Pediatric Surgery[ISSN:1671-6353/CN:43-1380/R]
volume:
第17卷
Number:
2018 05
Page:
383-389
Column:
实验研究
Date of publication:
2018-05-28
- Keywords:
- Short Bowel Syndrome; Immunohistochemistry; Rats; Animals; Laboratory
- Document code:
- A
- Abstract:
- ObjectiveTo investigate the contractile functions and Immunohistochemical characteristics of remnant ileum after intestinal adaptation in rats with short bowel syndrome.Methods The model of short bowel syndrome(SBS)was created by using surgical induction method.20 Sprague Dawley rats were divided into 2 groups in which they underwent intestinal resection and anastomosis or a sham surgery respectively.The morphological changes were observed by HE staining and AZAN staining;The variations of intestinal neurons,nerve fibers,ICC cells and smooth muscle cells were investigated by Immunohistochemistry.And the contractility of the ileum strips was measured by the organ-bath experiment.ResultsThere was no significant difference in weight between the two groups before the experiment.1 week after the operation,the weight of the SBS group and the control group was (264.83±64.30)g and(319.50±42.89)g respectively,and there was a statistical difference between the two groups（t=-2.292,P=0.033）.After 2 weeks of surgery,the weight of the SBS group and the control group was （317.71±63.50）g and（355.10±36.11）g respectively,but there was no statistical difference between the two groups（t=-1.672,P=0.109）.2 weeks after surgery,the perimeter of the proximal bowel and the distal bowel of SBS group rats was（28.88±2.71） mm and（19.65±1.66） mm respectively,with the control group （14.50±1.50）mm and（15.10±3.10）mm,respectively,and there were statistical significance (t=15.346,P<0.001; t=4.006,P=0.002).2 weeks after surgery,the length of the intestinal tube between SBS rats and the control group was（56.19±2.97）mm and（52.10±2.10)mm respectively,and there was a statistical significance(t=8.329,P<0.001).HE staining results indicated that the smooth muscle layer of the intestinal wall of the SBS group and the control group was（124.38±56.01）μm and（64.75±26.81）μm,and there was a statistical difference between the two groups(t=6.789,P<0.001）.The length of villi of SBS group and control group was （488.16±123.31） μm and（311.63±67.68） μm,and there was a statistical difference between the two groups( t=4.884,P<0.001).The crypt depth of SBS group and control group were（164.28±42.31）μm and（122.69±19.92）μm respectively,and there was a statistical difference between these two groups (t=5.226,P<0.001).The results of AZAN staining indicated that some muscle fibers in SBS group were replaced by collagen.The immunohistochemistry results indicated that the number of neurons and nerve fibers in the enteric nervous system of rats with the hyperplasia of the rats was more than that of the control group,and the ICC cells were decreased and the smooth muscle hypertrophy was obvious.Organ bath experiment hinted the strength of the SBS group of smooth muscle activity of rats significantly weaker than the control group at low concentration (10-7 mol/L,10-6 mol/L,10-5 mol/L) after acetylcholine stimulation,two groups had statistical significance (t value and P values of three groups of different concentrations of t=3.465,P=0.001;t=3.312,P=0.002;t=2.080,P=0.042).But at high concentration (10-4 mol/L),there was no statistical significance (t=1.782,P=0.083).ConclusionThe circular muscle motility of SBS showed normal but weaker pattern,and SBS cause increased neurons but impaired response to parasympathetic stimuli.SBS also cause some cases with reduced distribution of ICC.SBS cause some cases of muscular changes and replaced by collagen.Both of the structure adaptation and function adaptation met the needs of metabolism and growth of the body,in that many of them could gradually recovered from intestinal failure.
Last Update:
2018-05-23