Tong Ling,Zang Jingyu,Yuan Xiaojian,et al.Variation of RhoA/ROCK signaling pathway in colon of Hirschsprungs disease.[J].Journal of Clinical Pediatric Surgery,,17():226-230.
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Variation of RhoA/ROCK signaling pathway in colon of Hirschsprungs disease.

Journal of Clinical Pediatric Surgery[ISSN:1671-6353/CN:43-1380/R] volume: 第17卷 Number: 2018 03 Page: 226-230 Column: 实验研究 Date of publication: 2018-03-28
Author(s):
Tong Ling1; 2; 3; Zang Jingyu2; Yuan Xiaojian3; Hong Zhihua3; Wu Wenjie2; 3 Chen Jie2; 3; Xu Wenxie1; 2.
1.Department of Anatomy & Physiology，Shanghai Jiaotong University School of Medicine，Shanghai 200240，China；
Keywords:
Hirschsprung Disease;  Rho Kinase Signaling Pathway;  Myocytes;  Smooth Muscle;  Constriction;  Colon
Document code:
A
Abstract:
ObjectiveTo explore the protein expression of RhoA/ROCK signaling pathway in Hirschsprungs disease (HD) and provide new perspectives for studying colonic dysfunction in HD patients.MethodsThe protein expression involved in RhoA/ROCK signaling pathway was examined by Western blot in 9 HD cases aged from 6 months to 2 years.And statistical software was used for analyzing the results.ResultsThe expression of P(Ser19)MLC20 obviously decreased in narrow segment as compared with dilated counterpart (t=2.527，P=0.035)；and regarding the protein expressions involved in RhoA/ROCK signaling pathway，the expression of ROCK1 obviously decreased in narrow segment as compared with dilated counterpart (t=3.104，P=0.015).The expression of ROCK2 decreased in narrow segment compared with dilated counterpart (t=2.357，P=0.046).The expression of P(Thr853)MYPT1 also decreased in narrow segment as compared with dilated counterpart (t=2.857，P=0.028).And both had statistical significance.ConclusionRhoA/ROCK signaling pathway is down-regulated in HD.And these results may be related to colonic dysfunction of HD patients.
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