Wang Tianyi,Zhang Weiping,Han Wenwen,et al.Expression and significance of platelet-derived growth factor receptor alpha-positive cells in stenotic tissue of congenital ureteropelvic junction obstruction[J].Journal of Clinical Pediatric Surgery,2020,19(03):213-217.[doi:10.3969/j.issn.1671-6353.2020.03.005]
血小板源性生长因子受体α阳性细胞在先天性肾盂输尿管连接部梗阻发病中的表达及意义
- Title:
- Expression and significance of platelet-derived growth factor receptor alpha-positive cells in stenotic tissue of congenital ureteropelvic junction obstruction
- 关键词:
- 受体; 血小板源生长因子α; 肾盂输尿管连接部梗阻
- 分类号:
- R726.9;R692.7
- 摘要:
- 目的 探讨血小板源性生长因子受体α（platelet-derived growth factor receptor alpha-positive，PDGFRα）阳性细胞在儿童先天性肾盂输尿管连接部梗阻（ureteropelvic junction obstruction，UPJO）发病中的表达与意义。方法 应用免疫组织荧光双染方法对首都医科大学附属北京儿童医院30例UPJO患儿肾盂输尿管连接部（ureteropelvic junction，UPJ）组织（为UPJO组）及10例因肾脏肿瘤行肾输尿管切除术患儿的UPJ组织（排除肾盂输尿管连接部狭窄以及其他泌尿系统畸形，病理结果证实肿瘤未侵犯肾盂输尿管交界处，为对照组）进行染色观察，同时分析所有患儿PDGFRα阳性细胞表达的小电导钙离子激活钾离子通道3型（small conductance calcium-activated potassium channel 3，SK3）表达水平。结果 PDGFRα阳性细胞表型区别于Cajal间质细胞，与平滑肌细胞、神经纤维关系密切。PDGFRα细胞在UPJO组中的分布与对照组相似，而该细胞表达的SK3通道在UPJO组中显著低于对照组。结论 UPJO患儿肾盂输尿管连接部PDGFRα细胞上SK3通道表达降低，推测SK3通道可能通过干扰UPJ蠕动参与UPJO发病过程。
- Abstract:
- Objective To observe the expression of platelet-derived growth factor receptor α-positive (PDGFRα+) cells in congenital ureteropelvic junction obstruction (UPJO) for exploring their role in the pathogenesis of congenital hydronephrosis in children.Methods UPJO group was composed of specimens of ureteropelvic junction (UPJ) harvested during pyeloplasty(n=30) while control group involved the specimens of normal ureter (nephrectomy for tumor; n=10).The immunofluorescence of specimens was examined by light microscopy and computerized image analysis for the expression and distribution of PDGFRα+ cells in each group.In addition,small conductance calcium-activated potassium channel 3 (SK3) and closely associated cells consisting of smooth muscle cells (SMCs),interstitial cells of Cajal (ICCs) and nerve fibers were examined.Results Located in close proximity to SMCs,ICCs and nerve fibers,PDGFRα+ cells expressed SK3 channels capable of mediating purinergic inhibitory neurotransmission in SMCs.The expression of SK3 channels decreased obviously in UPJ obstruction in PDGFRα+ cells as compared with controls.Conclusion There is a down-regulation of SK3 channels in UPJO in PDGFRα+ cells.And SK3 channels may be involved in the pathogenesis of UPJO by perturbing normal peristalsis of human UPJ.
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备注/Memo
收稿日期:2019-10-20。
基金项目:北京市自然科学基金（编号：7172073）；北京市医管局儿科协同发展专项子课题（编号：XTZD20180303）
通讯作者:张潍平,Email:zhangwpp@163.com