Wang Bingjie,Zhou Junmei,Li Lingling,et al.Muscle components of gubernaculum testis from high/low cryptorchidic children[J].Journal of Clinical Pediatric Surgery,2019,18(06):523-526,528.[doi:10.3969/j.issn.1671-6353.2019.06.017]
人类高低位隐睾睾丸引带内肌肉组分的探究
- Title:
- Muscle components of gubernaculum testis from high/low cryptorchidic children
- Keywords:
- Cryptorchidism/ED; Muscles/UL
- 分类号:
- R697+.22;R726.9;R322.7+4
- 摘要:
- 目的 探讨人类高位和低位隐睾患儿睾丸引带内肌肉组分与睾丸位置的关系。方法 收集隐睾患儿术中废弃的睾丸引带，以麻醉下未降睾丸的位置位于内环口以上的腹腔型隐睾为高位组（n=11），已出内环口但未出外环口的腹股沟管型为低位组（n=62），分别进行组织切片染色观察其组织构成。结果 11例高位组患儿均未观察到引带内肌肉或肌肉样结构；62例低位组患儿中43例可观察到横纹肌结构，且排列较分散。人类高位隐睾引带组织HE染色主要表现为较致密的结缔组织，含较多成纤维细胞、少量小血管，未见横纹肌组织结构；低位隐睾引带HE染色，则表现为混合性组织，可见较疏松排列的成纤维细胞、少量血管和较多纤维，可观察到横纹肌。高位引带Masson染色可见其组织中结构较致密，含有大量胶原纤维成分和少量小血管；低位引带Masson染色可见疏松的胶原纤维成分、小血管、横纹肌结构。横纹肌特异性免疫荧光MY-32染色在高位组未观察到阳性信号，低位组中可见明显横纹肌结构。结论 人类隐睾睾丸引带在高位与低位时其组织肌肉成分存在差异，高位隐睾睾丸引带未见肌肉成分，低位隐睾睾丸引带中存在横纹肌。
- Abstract:
- Objective To explore the relationship between muscle components in gubernaculum testis and different positions of undescended testis in cryptorchidic children. Methods Gubernaculum testis was harvested from cryptorchidic children during orchidopexy.And the samples were divided into high and low groups according to the position of undescended testis under anesthesia.If the position of undescended testis was higher than that of inner ring,the sample was classified as high group (n=11).Contrarily,the position between inner ring and outer ring was designated as low group (n=62).Gubernaculum tissues were observed and compared by different stains of histological sections.Results No muscle or muscle-like structure was found in high group while striated muscle structures with scattered arrangement in 43/62 patients of low group.After hematoxylin-eosin staining,gubernaculum testis of high group was characterized largely by dense connective tissue,numerous fibroblasts and few small blood vessels without striated muscle tissue structure.However,tissues of low group showed mixed components.Striated muscle was composed of loosely arranged fibroblasts,few blood vessels and some fibers.After Masson’s staining,tissue structure was dense with collagen fibers and few small blood vessels in high group.Contrarily,loose collagen fiber components,small blood vessels and striated muscle structure were present in low group.After striated muscle-specific immunofluorescent MY-32 staining,no positive signal was detected in high group while obvious striated muscle structure existed in low group. Conclusion The muscle composition in gubernaculum of cryptorchidic children is different between high and low position of undescended testis.Striated muscle exists in human gubernaculum testis of low group but not in high group.
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备注/Memo
收稿日期:2019-03-20。
基金项目:国家自然科学基金（编号：81870459/81470911）；上海交通大学"医工交叉"基金（编号：YG2016MS32）
通讯作者:陈方,Email:doctorchenfang@126.com