Gao Runnan,Zhu Haitao,Shen Chun.Research advances in models of necrotizing enterocolitis in vivo and in vitro[J].Journal of Clinical Pediatric Surgery,2022,21(04):331-335.[doi:10.3760/cma.j.cn101785-202009024-007]
坏死性小肠结肠炎体内外模型的研究进展
- Title:
- Research advances in models of necrotizing enterocolitis in vivo and in vitro
- Keywords:
- Enterocolitis; Necrotizing; Models of Necrotizing Enterocolitis; Organoids; Epithelial Cells; Models; Animal; Intestines
- 摘要:
- 坏死性小肠结肠炎(necrotizing enterocolitis,NEC)是早产儿胃肠外科最常见急症之一。在过去50年里,为了研究该病的病理生理学特点以及明确新治疗策略的有效性,多种多样的实验模型应运而生。目前对于NEC的病因研究主要采用肠上皮细胞和NEC动物模型,然而细胞模型无法还原患儿肠组织体内生物学特性,动物模型无法反映人体疾病的相关特性。近年来肠道干细胞标记物Lgr5+的发现,使新型肠道体外模型——肠道类器官模型得以逐渐兴起。本文将就上述NEC体内外实验模型的研究进展进行综述,分析每种模型的特点与优劣势,进而帮助研究人员理解和选择与研究目的相关的NEC实验模型。
- Abstract:
- Necrotizing enterocolitis (NEC) has been a leading cause of gastrointestinal emergencies in premature infants.Over the last 50 years,a large variety of experimental models have been developed for examining the pathophysiology of NEC and testing the effectiveness of new therapeutic strategies.Current etiological studies of NEC have employed intestinal epithelial cells and animal models.However,cell models cannot mimic the biological characteristics of intestinal tissues in children while animal models reflect human diseases and other related characteristics.With the latest discovery of intestinal stem cell marker Lgr5+,a novel in vitro intestinal model is gradually emerging.This review focused upon the relevant experimental models and discussed the main advantages and disadvantages of each model so as to help researchers better select proper NEC experimental models.
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备注/Memo
收稿日期:2020-09-14;改回日期:。
基金项目:国家自然科学基金面上项目(81873849);上海市自然科学基金面上项目(22ZR1408600);国家儿童医学中心青年临床科学家项目(EK112520180306)
通讯作者:沈淳,Email:chunshen@fudan.edu.cn