Zhu Weiwen,Sun Jie.Correlations of the expression of androgen receptor in appendix testis with undescended testis and testis development[J].Journal of Clinical Pediatric Surgery,2021,20(08):754-760.[doi:10.12260/lcxewkzz.2021.08.010]
睾丸附件雄激素受体表达与睾丸下降及隐睾发育情况的相关性分析
- Title:
- Correlations of the expression of androgen receptor in appendix testis with undescended testis and testis development
- Keywords:
- Cryptorchidism; Appendix Testis; Receptors; Androgen
- 分类号:
- R726.972.2;R726.9
- 摘要:
- 目的 通过对隐睾患者的睾丸附件雄激素受体进行分析，探究睾丸附件的雄激素受体表达与睾丸下降及睾丸发育之间的潜在关系。方法 收集2019年11月至2020年5月由上海交通大学医学院附属上海儿童医学中心收治的39例隐睾患者的临床资料，对术中切除的睾丸附件标本进行免疫组化检查，并将隐睾患者分为先天性隐睾（n=28）与获得性隐睾（n=11），进一步根据睾丸位置，将患者分为腹腔型隐睾（n=7）、腹股沟上段型隐睾（n=25）和腹股沟下段型隐睾（n=7）三组。本研究同时收集患者睾丸术前超声检查相关参数，对不同位置的睾丸，单侧或双侧隐睾的睾丸附件雄激素受体表达进行光密度半定量分析，并运用统计学方法分析隐睾患者睾丸长径与睾丸附件雄激素受体表达水平的相关性。结果 先天性双侧隐睾患者的睾丸附件雄激素受体表达阳性率为30.7%（4/13），而单侧隐睾和获得性隐睾患者的睾丸附件雄激素受体阳性率为100%，差异有统计学意义（P<0.001）。在表达雄激素受体的隐睾患者中，腹腔型隐睾患者的雄激素受体（androgen receptor，AR）平均光密度的中位数为22×10-4（2×10-4，154×10-4），腹股沟上段型隐睾为115×10-4（46×10-4，315×10-4），差异有统计学意义（P=0.045）。同时，隐睾的患侧睾丸长径与对应睾丸附件AR平均光密度值具有线性关系（Rsup>2=0.264，P=0.035）。结论 隐睾患者睾丸附件雄激素受体的表达水平可能与睾丸位置以及睾丸的发育情况相关。双侧隐睾的发生可能与激素的关系紧密，通过对隐睾睾丸附件AR的研究，能在一定程度上反映隐睾的发生机制。本研究提示了睾丸附件雄激素受体表达与睾丸下降及睾丸发育有相关性，为理解隐睾的发生机制提供给了新的思路。
- Abstract:
- Objective To explore the expression of androgen receptor in appendix testis in cryptorchidism and examine a possible relation between androgen receptor and testis descend and testis development. Methods Clinical data were collected from cryptorchidism patients from November 2019 to May 2020. Appendix testis was collected from hospitalized children of orchipexy. They were divided into abdominal testis, upper inguinal and lower inguinal testis according to the location of testis. The preoperative results of scrotum ultrasound were collected. The average optical density was calculated and statistic analysis performed for evaluating the relation between testis length and the expression level of androgen receptor in appendix testis. Results All androgen receptors in appendix testis were positive in acquired cryptorchidism children and it was merely 30.7% positive in congenital bilateral cryptorchidism counterparts (P<0.001). In those with positive androgen receptor, abdominal testis group had a mean optical density of 22×10-4(2×10-4, 154×10-4), and it was 115×10-4(46×10-4, 315×10-4) in upper inguinal testis group. Moreover, the length of affected side of testis had a positive correlation with the average optical density in appendix testis. Conclusion The expression level of androgen receptor in appendix testis is probably correlated with testis location/ development. Bilateral cryptorchidism has a close relation with hormone. Examining androgen receptor in appendix testis help researchers understand the descending of testis. This study hints at a possible relation between testis descend and appendix testis. It may facilitate a mechanistic understanding of cryptorchidism in the future.
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备注/Memo
收稿日期:2020-08-14。
基金项目:国家自然科学基金青年项目（编号：81900618）；上海"科技创新行动计划"扬帆计划（编号：18YF1415000）
通讯作者:孙杰,Email:ppdrsun@gmail.com