Xing Xiaoyu,Chen Siping,Zhong Liang,et al.A preliminary study on the relationship between vitamin D and its receptor and ureteropelvic junction obstruction[J].Journal of Clinical Pediatric Surgery,2020,19(03):209-212.[doi:10.3969/j.issn.1671-6353.2020.03.004]
维生素D及其受体与肾盂输尿管连接部狭窄相关性的初步研究
- Title:
- A preliminary study on the relationship between vitamin D and its receptor and ureteropelvic junction obstruction
- 关键词:
- 维生素D; 肾盂输尿管连接处狭窄; 纤维化
- Keywords:
- Vitamin D; Ureteropelvic Junction Obstruction; Fibrosis
- 分类号:
- R726.9;R692.7;R977.2+4
- 摘要:
- 目的 初步分析维生素D及其受体与肾盂输尿管连接部狭窄（ureteropelvic junction obstruction，UPJO）发生的相关性。方法 选择2016年3月1日至2016年10月31日上海交通大学医学院附属上海儿童医学中心小儿泌尿外科收治的因UPJO行离断式肾盂输尿管成形术的患儿为UPJO组（n=10），因肾脏肿瘤行肾与输尿管切除术的患儿为对照组（n=8）。收集两组术前血标本检测维生素D三项（25-羟维生素D2、25-羟维生素D3及总25-羟维生素D的含量）。取两组术中标本采用ELISA法，检测维生素D受体（vitamin D receptor，VDR）、转化生子因子（transforming growth factor-β，TGF-β）及钙黏蛋白E的含量。结果 两组术前血标本中25-羟维生素D2、25-羟维生素D3及体内总25-羟维生素D的含量均无统计学差异（P>0.05）。UPJO组VDR、TGF-β、钙黏蛋白E的平均值分别为（771.6±70.57）ng/每克总蛋白，（131.0±10.33）ng/每克总蛋白及（125.5±10.98）ng/每克总蛋白；对照组分别为（2 128±251.8）ng/每克总蛋白，（82.3±6.834）ng/每克总蛋白及（386.6±78.71）ng/每克总蛋白。两组UPJ标本中VDR、TGF-β、钙黏蛋白E水平的差异有统计学意义（P<0.05）。结论 UPJO组VDR表达量显著低于对照组，提示VDR可能与UPJO发生相关，初步推测其机制可能是肾盂输尿管连接部VDR表达量减少引起TGF-β表达量明显升高，使得肾盂输尿管连接部的组织纤维化加重，进而导致或加重UPJO。
- Abstract:
- Objective To explore the correlation between UPJO and vitamin D and its receptor.Methods 10 cases of children who underwent UPJO disconnection pyeloplasty were divided into UPJO group.8 children who underwent renal and ureterectomy for renal tumors were enrolled in the control group.The preoperative contents of 25 hydroxyvitamin D2,25 hydroxyvitamin D3 and total 25 hydroxyvitamin D were detected.VDR,TGF-β and cadherin E content were detected by ELISA staining.The results of the test were used t test for statistical analysis,compare the differences between two groups,and analyze the correlation between vitamin D and its receptor and UPJO.Results there was no significant difference between the two groups in the detection of vitamin D three items before operation.The mean values of VDR,TGF-β and cadherin E in UPJO group were 771.6±70.57 ng/g,131.0±10.33 ng/g and 125.5±10.98 ng/g respectively.The corresponding mean values in tumor control group were 2128±251.8,82.3±6.834 and 386.6±78.71 ng/g respectively.The data of two groups were tested by t-test (P<0.05).The expression of VDR and cadherin E in UPJO group was significantly lower than that in control group.TGF-β was significantly higher than that of the control group.Conclusion The expression of VDR receptor in UPJO group was significantly lower than that in control group,suggesting that VDR may be related to UPJO.The initial mechanism may be the decrease in the expression of VDR at UPJ,causing a significant increase in the expression of TGF-β,which aggravates the tissue fibrosis at the UPJ,resulting in or aggravating UPJO.
参考文献/References:
1 Bover J,Egido J,Fernández-Giráldez E,et al.Vitamin D,vitamin D receptor and the importance of its activation in patients with chronic kidney disease[J].Nefrologia,2015,35(1):28-41.DOI:10.3265/Nefrologia.pre2014.Sep.11796.
2 Ellerkamp V,Kurth RR,Schmid E,et al.Differences between intrinsic and extrinsic ureteropelvic juncti,Kurth RRon obstruction related to crossing vessels:histology and functional analyses[J].World J Urol,2016,34(4):577-583.DOI:10.1007/s00345-015-1645-x.
3 Zerr P,Vollath S,Palumbo-Zerr K,et al.Vitamin D receptor regulates TGF-beta signalling in systemic sclerosis[J].Ann Rheum Dis,2015,74(3):e20.DOI:10.1136/annrheumdis.2013-204378.
4 Lucisano S,Buemi M,Passantino A,et al.New insights on the role of vitamin D in the progression of renal damage[J].Kidney Blood Press Res,2013,37(6):667-678.DOI:10.1159/000355747.
5 孙劲松,刘丹丹,张刚,等.先天性肾盂输尿管连接部梗阻的临床病理学分析[J].临床小儿外科杂志,2018,17(6):429-432.DOI:10.3969/j.issn.1671-6353.2018.06.007. Sun JS,Liu DD,Zhang G,et al.Clinicopathological study of congenital ureteropelvic junction obstruction[J].J Clin Ped Sur,2018,17(6):429-432.DOI:10.3969/j.issn.1671-6353.2018.06.007.
6 朱冬燕,孙奕,孙楠,等.活性维生素D3抑制UUO模型大鼠肾间质纤维化作用的实验研究[J].中国中西医结合肾病杂志,2013,14(3):207-211.DOI:10.3969/j.issn.1009-587X.2013.03.006. Zhu DY,Sun Y,Sun N,et al.Study on effect of 1,25(OH)2D3 on inhibiting the renal interstitial fibrosis in rats following unilateral ureteral obstruction[J].Chinese Journal of Integrated Traditional and Western Nephrology,2013,14(3):207-211.DOI:10.3969/j.issn.1009-587X.2013.03.006.
7 张国锐,林智峰,杨晓萍,等.1,25-(OH) 2D3对单侧输尿管梗阻模型大鼠Erbin表达的影响[J].现代医药卫生,2016,32(17):2650-2651.DOI:10.3969/j.issn.1009-5519.2016.17.010. Zhang GR,Lin ZF,Yang XP,et al.Effect of 1,25-(OH)2D3 on Erbin expression in unilateral ureteral obstruction rat model[J].J Mod Med Health,2016,32(17):2650-2651.DOI:10.3969/j.issn.1009-5519.2016.17.010.
8 Zittermann A,Ernst JB,Gummert JF,et al.Vitamin D supplementation,body weight and human serum 25-hydroxyvitamin D response:a systematic review[J].Eur J Nutr,2014,53(2):367-374.DOI:10.1007/s00394-013-0634-3.
9 Meng XM,Nikolic-Paterson DJ,Lan HY.TGF-beta:the master regulator of fibrosis[J].Nat Rev Nephrol,2016,12(6):325-338.DOI:10.1038/nrneph.2016.48.
10 Merrikhi A,Bahraminia E.Association of urinary transforming growth factor-beta1 with the ureteropelvic junction obstruction[J].Adv Biomed Res,2014,33,123.DOI:10.4103/2277-9175.133196.eCollection 2014.
11 刘艳姝,刘文思,刘中柱, 等.活性维生素D3抑制UUO模型大鼠肾间质纤维化作用的研究及其对TGF-β1,α-SMA表达的影响[J].黑龙江医药科学,2013,36(2):72-73.DOI:10.3969/j.issn.1008-0104.2013.02.038. Liu YS,Liu WS,Liu ZZ,et al.Suppression of active vitamin D3 on renal interstitial fibrosis in UUO model rats and its effect on expression of TGF-1 or α-SMAJ[J].Heilongjiang Medicine and Pharmacy,2013,36(2):72-73.DOI:10.3969/j.issn.1008-0104.2013.02.038.
12 Tian Y,Lv G,Yang Y,et al.Effects of vitamin D on renal fibrosis in diabetic nephropathy model rats[J].Int J Clin Exp Pathol,2014,7(6):3028-3037.
相似文献/References:
[1]朱彤,李连永,刘天婧,等.早产儿维生素D及骨代谢指标水平的相关性研究[J].临床小儿外科杂志,2020,19(05):420.[doi:10.3969/j.issn.1671-6353.2020.05.009]
Zhu Tong,Li Lianyong,Liu Tianjing,et al.Correlations between prematurity and vitamin D and bone metabolic markers in neonates[J].Journal of Clinical Pediatric Surgery,2020,19(03):420.[doi:10.3969/j.issn.1671-6353.2020.05.009]
备注/Memo
收稿日期:2018-05-22。
基金项目:国家自然科学基金（编号：81871149），国家自然科学基金（编号：81601267）
通讯作者:孙杰,Email:sunjie@scmc.com.cn