[1]李明勇,王星,石秦林,等.Ｓｍａｄ７，Ｓｍａｄ３和Ｓｍａｄ２在ＤＥＨＰ诱导的尿道下裂大鼠阴茎中的表达[J].临床小儿外科杂志,2018,17(02):136-140.
　Li Mingyong,Wang Xing,Shi Qinlin,et al.Exprssion of Smad7,Smad3 and Smad2 in the hypospadiac penis of fetal rats induced by DEHP.[J].Journal of Clinical Pediatric Surgery,2018,17(02):136-140.
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Ｓｍａｄ７，Ｓｍａｄ３和Ｓｍａｄ２在ＤＥＨＰ诱导的尿道下裂大鼠阴茎中的表达

《临床小儿外科杂志》[ISSN:1671-6353/CN:43-1380/R] 卷: 第17卷 期数: 2018年02期 页码: 136-140 栏目: 实验研究 出版日期: 2018-02-28
Title:
Exprssion of Smad7,Smad3 and Smad2 in the hypospadiac penis of fetal rats induced by DEHP.
作者:
李明勇; 王星; 石秦林; 等.
南华大学附属第一医院泌尿外科科室
Author(s):
Li Mingyong1; Wang Xing1; Shi Qinlin2; Wei Guanghui2; Cao Youhan1; Deng Xiang1; Xu Hanfeng1; Li Qing1.
1.Department of Urology Surgery,First Affiliated Hospital,University of South China,Hengyang 421001,China.
关键词:
尿道下裂; 二乙基己基邻苯二甲酸; Ｓｍａｄ７蛋白质; Ｓｍａｄ３蛋白质; Ｓｍａｄ２蛋白质; 大鼠
Keywords:
Hypospadias;  Diethylhexyl Phthalate;  Smad7 Protein;  Smad3 Protein;  Smad2 Protein;  Rats
文献标志码:
A
摘要:
目的　研究Ｓｍａｄｓ（７，３和２）在邻苯二甲酸二（２乙基）己酯［ｄｉ（２ｅｔｈｙｌｈｅｘｙｌ）ｐｈｔｈａｌａｔｅ，
（ＤＥＨＰ）］诱导的尿道下裂大鼠阴茎组织中的表达变化，探讨尿道下裂发生机制。　方法　将ＳＤ孕鼠
随机分为２组：实验组（将ＤＥＨＰ溶于１．５ｍＬ大豆油中灌胃大鼠）和对照组（以１．５ｍＬ大豆油灌胃大
鼠），每组２０只，自孕１２ｄ（ｇｅｓｔａｔｉｏｎｄａｙ，ＧＤ１２）至１９ｄ（ＧＤ１９）连续每天定时给药１次。至ＧＤ２０行剖
宫产取出雄性胎鼠，切取阴茎组织，采用实时定量ＰＣＲ和免疫组化法分别检测胎鼠阴茎中Ｓｍａｄ７，
Ｓｍａｄ３和Ｓｍａｄ２三者ｍＲＮＡ和蛋白质表达水平。　结果　经ｑＰＣＲ检测分析后发现，Ｓａｍｄ７，Ｓｍａｄ３和
Ｓｍａｄ２三者ｍＲＮＡ在正常对照组相对于内参ＧＡＰＤＨ的表达分别是１．００，０．８４和１．１４，在ＤＥＨＰ暴露
组其相对表达分别是１．８７，１．３６和１．４９，且三者在实验组的表达与正常对照组比较，均升高（Ｐ值分别
为＜０．００１，０．０４１和０．０２１）；观察实验组Ｓｍａｄ７和ｐＳｍａｄ２／３蛋白表达水平亦有增加趋势。　结论　
ＤＥＨＰ诱导的先天性尿道下裂可能与胎鼠阴茎组织中Ｓｍａｄｓ（７，３和２）的过度表达有关。
Abstract:
ObjectiveTo investigate the expression of Smads (7,3 and 2) in the hypospadias penis of fetal rats induced by maternal exposure to di(2ethylhexyl) phthalate (DEHP),in order to explore the etiology of hypospadias.MethodsPregnant rats were randomly divided into experiment group and control group,20 rats for each group.Each rat in the experiment group was treated with DEHP dissolved in 1.5ml soybean oil by savaging,while control group were treated with 1.5ml soybean oil,once a day from the 12th gestation days (GD12) to GD19.Fetal rats were obtained on the GD20 by caesarean section,and were investigated for the mRNA and protein expression levels of Smad7,Smad3 and Smad2 by using realtime quantitative PCR and immunohistochemistry assay respectively.ResultsThe expression of Smad7,Smad3 and Smad2 mRNA in hypospadias rats foreskin tissue increased 97.0%,22.8% and 59.9% times compared with normal control groups,respectively,and the differences were significant (all P<0.05).Moreover,the protein levels of Smad7 and phosphorylated Smad 2/3 in foreskin from children with hypospadias showed an incremental trend.ConclusionOur results preliminary indicate that Smads(7,3 and 2) overexpression might be associated with the DEHPinduced congenital hypospadias.

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