GU Tao,LU Hong-ting,DONG Qian.The relationship between Kai1/CD82 expression and metastasis of neuroblastoma[J].Journal of Clinical Pediatric Surgery,2013,12(02):100-102.[doi:10.3969/j.issn.1671— 6353.2013.02.007]
Kai1/CD82表达水平与神经母细胞瘤转移的关系探讨
- Title:
- The relationship between Kai1/CD82 expression and metastasis of neuroblastoma
- Keywords:
- Neuroblastoma; Tumor Suppressor Genes; Kai1/CD82
- 摘要:
- 目的 探讨Kai1/CD82表达水平与神经母细胞瘤转移之间的关系。 方法 选取40例各期神经母细胞瘤石蜡标本,采用RT-PCR检测Kai1mRNA的表达; 采用免疫组化检测Kai 1基因的蛋白产物CD82的表达水平,研究其与神经母细胞瘤转移之间的关系。 结果 RT-PCR监测显示:有淋巴结或远处转移的神经母细胞瘤Kai1mRNA的相对含量为0.0748±0.0063,而无转移者为0.4576±0.0226,差异有统计学意义(P<0.05)。免疫组化显示:无转移的神经母细胞瘤CD82的阳性表达率为71.43%; 而有淋巴结或远处转移者阳性表达率为31.58%,差异有统计学意义(P<0.05),有骨髓浸润者与无骨髓浸润者Kai1/CD82的表达无统计学意义(P>0.05)。 结论 Kai1/CD82表达水平可作为判断神经母细胞瘤是否转移的一个潜在标志
- Abstract:
- Objetive To investigate the relationship between Kai1 / CD82 expression and metastasis of neuroblastoma. Methods RT-PCR shall be applied to test the expression of Kai1mRNA in 40 neuroblastoma paraffin specimens of each period.Kai1/CD82 level was measured by immunohistochemistry method in 40 neuroblastoma paraffin specimens of each period. Results RT-PCR shows that the relative content of Kai1mRNA in in the neuroblastoma group with lymph node or distant metastasis is 0.0748±0.0063,the relative content of Kai1mRNA in the neuroblastoma group without metastasis is 0.75±0.03, there is statistical significance in the two group difference.The positive rate of Kai1/CD82 expression was 71.43% in the neuroblastoma group without metastasis and 31.58% in the neuroblastoma group with lymph node or distant metastasis. There was a significant difference in the Kai1/CD82 expression between neuroblastoma groups with and without metastasis. There was no significant differences in the Kai1/CD82 expression between metastasis neuroblastoma groups with and without bone marrow infiltration. Conclusions The expression level of Kai1/CD82 can be used as a potential predictor of metastasis of neuroblastoma.
参考文献/References:
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