Comparison of two different methods to establish animal model of hydronephrosis due to incomplete ureteral obstruction.[J].Journal of Clinical Pediatric Surgery,2011,10(02):107-110.
两种输尿管不全梗阻肾积水动物模型制作方法的比较
- Title:
- Comparison of two different methods to establish animal model of hydronephrosis due to incomplete ureteral obstruction.
- Keywords:
- ; Ureteral Obstruction; Uronephrosis; Modela; Animal
- 文献标志码:
- A
- 摘要:
- 【摘要】目的比较两种输尿管不全梗阻致肾积水动物模型制作方法的优劣。方法将10周龄雄性新西兰兔24只随机分为腰大肌组和塑料套管组，每组12只，分别应用腰大肌包埋输尿管法及塑料套管压迫法制作右侧肾盂输尿管连接部梗阻动物模型。模型建立前后10周分别对两组样本行核医学肾动态显像及CT灌注扫描，测得肾小球滤过率（GFR）、肾实质厚度、肾盂前后径、肾皮质及髓质的血容量（BV）、血流量（BF）、平均通过时间（MTT）及表面通透性（PS） 等，并以血肌酐、尿素氮、B超及病理检查为佐证，观察两组样本各项参数的变化，进行统计学分析。结果两组样本右侧肾脏均呈慢性肾积水表现，血肌酐、尿素氮较术前升高，两组右肾GFR、肾实质厚度以及CT灌注参数BV、BF、PS均较术前下降，但套管组下降更为明显（P<0.05）；两组右肾肾盂前后径较术前增大，套管组增大更为明显（P<005）。结论两种方法制作肾盂输尿管连接部梗阻模型均具可行性。但塑料套管压迫法制作模型较为稳固，肾积水效果显著，相比于腰大肌包埋输尿管法具有一定的优势，可作为肾积水模型的优先选择方法。
- Abstract:
-
【Abstract】ObjectiveTo establish and compare two different methods of hydronephrosis due to ureteropelvic junction obstruction in rabbit.MethodsTwentyfour New Zealand rabbits （10weekold，Male） were randomly assigned to two groups: group A （12 rabbits） were embedded the right ureter of ureteropelvic junction under the psoas major muscle and group B（12 rabbits） were emboliaed the right ureter of ureteropelvic junction into a plastic cannula. All rabbits were performed both on SPECT and CT perfusion preoperative and postoperative respectively, and parameters including GFR, thickness of renal cortical, diameter of pelvis, BV, BF, MTT and PS were assessed and the statistical significance of differences was determined, in terms of BUN、CR、ultrasound result、pathologic diagnoses pathology result and statistical analyses were performed simultaneously on the GFR of SPECT and CT perfusion parameters.ResultsThe success rate of animal model was 83.3% in group A and 91.7% in group B, respectively. All models presented chronic hydronephrosis by pathologically confirmed. BUN and CR of right kidney both group A and group B were increased after operation, Perfusion parameters including BV、BF、PS and GFR of both group A and group B were decreased but rate of decline of group A was more significant than group B （P<0.05）. Both of group showed dilation of renal calyx and pelvis, as well as there was statistically significant difference between the two sets（P＜0.05）.ConclusionsBoth two method provide highly feasibility for hydronephrosis in rabbit model , but we would recommend method of plastic cannula is the best and first the choice.
参考文献/References:
1马胜利,叶章群,陈志强.输尿管部分梗阻后肾小球滤过率的时相性变化研究\[J\].武汉大学学报（医学版）,2006,27（3）:399—402.
2周显礼,李昭铸,王晓蕾,等.小儿肾积水血流动力学观察及临床意义\[J\].中国临床医学影像杂志,2004,15（4）:202—204.
3黄澄如.实用小儿泌尿外科学\[M\].北京:人民卫生出版社.2006,198—209.
4吕宇涛,文建国,张艳，等.肾脏血流阻力指数比率在评估小儿肾积水肾脏功能中的价值\[J\].郑州大学学报（医学版）,2003,38（2）: 182—184.
5何上进,张杰,张孝斌,等.肾内阻力指数对梗阻性积水肾功能的评估\[J\].临床外科杂志2005,13（6）:373—375.
6PerezBrayfield MR,Kirsch AJ, Jones RA,et al.A prospective study comparing ultrasound, nuclear scintigraphy and dynamic contrast-enhanced MRI for the evaluation of hydronephrosis\[J\].J Urol,2003,170:1330—1334.
相似文献/References:
[1]孙 邡
冯东川
朱孝宇.双J管在小儿小切口肾盂成形术中的应用[J].临床小儿外科杂志,2010,9(02):0.
[2]刘婷婷 张钦明 周红 焦丽丽 陈永卫. 肠内营养在儿童胰腺外伤治疗中的应用[J].临床小儿外科杂志,2011,10(02):84.
LIU Ting ting,ZHANG Qin ming,ZHOU Hong,et al. Enteral nutrition treatment in the application of pancreas trauma of children.[J].Journal of Clinical Pediatric Surgery,2011,10(02):84.
[3]吴卫卫 汪健 黄顺根. 肠外营养导致大鼠肝细胞炎症因子及受体基因表达的变化及临床意义[J].临床小儿外科杂志,2011,10(02):87.
Parenteral Nutrition Leads to Alteration of Inflammation Cytokines and Receptors Gene Expression in the Rat[J].Journal of Clinical Pediatric Surgery,2011,10(02):87.
[4]于增文 杨杨 李索林 耿娜 李英超 李萌 马亚贞. 腹腔镜下十二指肠单层缝合吻合术的实验研究与临床应用[J].临床小儿外科杂志,2011,10(02):90.
YU Zen gwen,YANG Yang,LI Suo lin,et al. The experimental studies and clinical applications of laparoscopic duodenal singlelayer sutured anastomosis in children.[J].Journal of Clinical Pediatric Surgery,2011,10(02):90.
[5]徐织 王剑 傅启华 杨波 宋云海 鲍南.中国人群神经管缺陷VANGL1基因突变的筛查及关联研究[J].临床小儿外科杂志,2011,10(02):94.
Mutation screening and associated study of VANGL1 gene in chinese patients with Neural Tube Defects.[J].Journal of Clinical Pediatric Surgery,2011,10(02):94.
[6]丁晋阳 莫绪明 顾海涛 孙剑 彭卫 戚继荣 沈立 束亚琴 钱龙宝. 延迟关胸技术在婴儿复杂危重先心病手术中的应用[J].临床小儿外科杂志,2011,10(02):98.
DING Jin yang,MO Xu ming,GU Hai tao,et al. Delayed Sternal Closure following operations for complex and severe congenital heart disease in infant[J].Journal of Clinical Pediatric Surgery,2011,10(02):98.
[7]吴臻斐 林孝坤 秦乐 林进汉 李仲荣 陈肖鸣. 西地那非对大鼠单侧睾丸扭转后对侧睾丸的影响及作用研究[J].临床小儿外科杂志,2011,10(02):101.
[J].Journal of Clinical Pediatric Surgery,2011,10(02):101.
[8]王贤书 张晓茹 杨志国 程征海 岳芳 曹红宾 李德泽. 脑室腹腔分流术治疗婴儿脑积水120例 [J].临床小儿外科杂志,2011,10(02):105.
[9]俞钢 朱小春 葛午平 林炎坤 金龙 劳伟华 史浩 肖尚杰 洪淳 周佳亮 傅晓静. 胎儿外科相关疾病的产前诊断及干预[J].临床小儿外科杂志,2011,10(02):111.
The antenatal diagnosis, tassessment, and therapy of fetal surgical anomalies[J].Journal of Clinical Pediatric Surgery,2011,10(02):111.
[10]刘国庆 王剑锋 朵永福 李杰荣 陈一平 黄振强 叶章群. 妊娠期尿结石对胎儿早产流产的影响[J].临床小儿外科杂志,2011,10(02):115.
Urolithiasis influence to develop of fetus in gestation period[J].Journal of Clinical Pediatric Surgery,2011,10(02):115.
[11]刘颖 毕允力. 气膀胱输尿管再植术治疗婴儿输尿管膀胱连接部梗阻临床分析[J].临床小儿外科杂志,2014,13(04):287.
[12]程时刚,陈海涛,耿红琼,等. Cajal细胞在先天性肾盂输尿管连接部梗阻中的分布及意义[J].临床小儿外科杂志,2015,14(06):506.