Qin Shuangli,Xie Chong,Zhou Ling,et al.Evaluation of matrix metalloproteinase-7 and matrix metalloproteinase-9 as biomarkers in ureteropelvic junction obstruction in children:a preliminary report[J].Journal of Clinical Pediatric Surgery,2021,20(07):664-667.[doi:10.12260/lcxewkzz.2021.07.013]
儿童肾盂输尿管连接部梗阻患者尿液中MMP-7和MMP-9的临床意义
- Title:
- Evaluation of matrix metalloproteinase-7 and matrix metalloproteinase-9 as biomarkers in ureteropelvic junction obstruction in children:a preliminary report
- 关键词:
- 基质金属蛋白酶7/分析; 基质金属蛋白酶9/分析; 肾盂积水; 肾盂输尿管连接部梗阻/诊断
- Keywords:
- Matrix Metalloproteinase 7/AN; Matrix Metalloproteinase 9/AN; Hydronephrosis/DI; Ureteropelvic junctin obstruction/DI
- 分类号:
- R726.921.7;R726.912
- 摘要:
- 目的 探讨尿液中MMP-7和MMP-9对儿童肾盂输尿管连接部梗阻（ureteropelvic junction obstruction，UPJO）的诊断价值。方法 本研究共纳入新疆维吾尔自治区人民医院24例单侧UPJO患者作为病例组，其中左侧18例、右侧6例，平均年龄5.8岁（37天至15岁），20例完成肾动态显像。16例行肾盂输尿管成形术（为手术组），余8例为非手术组；另纳入16例健康儿童作为对照组。随访时间为术后6个月至1年，随访项目包括超声、尿液MMP-7和MMP-9水平。结果 病例组尿中MMP-7/尿肌酐和MMP-9/尿肌酐水平高于对照组（P<0.05）；手术组MMP-7/尿肌酐和MMP-9/尿肌酐水平均高于非手术组（P<0.05）；10例获随访患者术前MMP-7/尿肌酐和MMP-9/尿肌酐水平均高于术后组（P<0.05）。不同肾积水程度患者的MMP-7/尿肌酐和MMP-9/尿肌酐水平值比较，差异无统计学意义（P>0.05）。MMP-7/尿肌酐、MMP-9/尿肌酐与肾GFR无相关性（r=-0.128，P=0.592；r=-0.157，P=0.507）。结论 与健康儿童相比，单侧UPJO患者尿液中MMP-7、MMP-9水平明显升高，行手术治疗者MMP-7/尿肌酐和MMP-9/尿肌酐水平显著高于非手术治疗者，术后6个月患者尿液中MMP-7、MMP-9水平较术前下降。
- Abstract:
- Objective To evaluate the application values of urinary matrix metalloproteinase-7 (MMP-7) and matrix metalloproteinase-9 (MMP-9) as biomarkers in children with ureteropelvic junction obstruction (UPJO).Methods A total of 24 children with unilateral UPJO were recruited along with another 16 healthy controls.The involved side was left (n=18) and right (n=6).And the average age was 5.8 years (37 days to 15 years).Among them,20 children received 99mTc-DTPA (diethylenetriaminepentaacetic acid) renal dynamic imaging and another 16 underwent pyeloplasty.Quantitative urinary protein analysis was performed and urinary creatinine level employed for normalizing protein levels for the biomarkers.Results The creatinine-normalized urinary levels of MMP-7/MMP-9 protein were higher in UPJO children than healthy controls (P<0.05).Moreover,urinary levels of MMP-7/MMP-9 protein were higher in operation group than those in non-operation group.The preoperative levels of MMP-7/MMP-9 protein in 10 children were higher than those post-operation (P<0.05).No significant differences existed in creatinine-normalized urinary levels of MMP-7/MMP-9 in varying degrees of hydronephrosis (P>0.05).And normalized levels of MMP-7/MMP-9 were not correlated with glomerular filtration rate of affected side (r=-0.128,P=0.592; r=-0.157,P=0.507).Conclusion Compared with healthy children,urinary levels of MMP-7/MMP-9 of children with unilateral UPJO were significantly higher.The levels of MMP-7/MMP-9 proteins were also significantly higher in operation group than those in non-operation group and the levels of MMP-7/MMP-9 protein declined during follow-ups at Month 6 post-operation.
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备注/Memo
收稿日期:2020-05-22。
基金项目:新疆维吾尔自治区自然科学基金资助项目基金（编号：2019D01C112）
通讯作者:谢崇,Email:1298147216@qq.com;周玲,Email:zhouling781004@163.com