[1]李艳云,刘雪来,崔钊,等.TLR4/TRIF信号参与Ⅰ型小肠闭锁隔膜组织黏膜免疫应答的组织学研究[J].临床小儿外科杂志,2023,22(02):180-184.[doi:10.3760/cma.j.cn101785-202202018-015]
　Li Yanyun,Liu Xuelai,Cui Zhao,et al.Histological observation of TLR4/MyD88 signaling pathway in septum arisen from type Ⅰ intestinal atresia[J].Journal of Clinical Pediatric Surgery,2023,22(02):180-184.[doi:10.3760/cma.j.cn101785-202202018-015]
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TLR4/TRIF信号参与Ⅰ型小肠闭锁隔膜组织黏膜免疫应答的组织学研究

《临床小儿外科杂志》[ISSN:1671-6353/CN:43-1380/R] 卷: 第22卷 期数: 2023年02 页码: 180-184 栏目: 实验研究 出版日期: 2023-02-28
Title:
Histological observation of TLR4/MyD88 signaling pathway in septum arisen from type Ⅰ intestinal atresia
作者:
李艳云1;  刘雪来2;  崔钊3;  高鹏4;  宋岩彪5
1 解放军总医院儿科医学部 解放军总医院第七医学中心儿科研究所 出生缺陷防控关键技术国家工程实验室 儿童器官功能衰竭北京市重点实验室, 北京 100700;
2 首都儿科研究所附属儿童医院外科, 北京 100020;
3 长春市儿童医院外科, 长春 130061;
4 哈尔滨市儿童医院外科, 哈尔滨 150010;
5 河北医科大学第二医院中心实验室, 石家庄 050000
Author(s):
Li Yanyun1;  Liu Xuelai2;  Cui Zhao3;  Gao Peng4;  Song Yanbiao5
1 Department of Neonatology, Bayi Children’s Hospital, Seventh Medical Center of PLA General Hospital, Beijing 100700, China;
2 Department of Surgery, Capital Institute of Pediatrics Affiliated Children’s Hospital, Beijing 100020, China;
3 Department of Surgery, Municipal Children’s Hospital, Chang Chun 130061, China;
4 Department of Surgery, Harbin Children’s Hospital, Harbin 150010, China;
5 Department of Central Labarotory, Second Hospital of Hebei Medical University, Shijiazhuang 050000, China
关键词:
肠闭锁; 病因学; 肠闭锁; 病理学; 隔膜组织; 免疫学; Toll样信号通路
Keywords:
Intestinal Atresia;  Etiology; Intestinal Atresia;  Pathology; Septum; Immunology; Toll-like Receptor Signaling Pathway
DOI:
10.3760/cma.j.cn101785-202202018-015
摘要:
目的 观察Ⅰ型小肠闭锁肠腔隔膜组织黏膜β干扰素TIR结构域衔接蛋白(TIR-domain-containing adapter-inducing interferon-β,TRIF)、肿瘤坏死因子受体相关因子蛋白3(tumor receptor associated factor,TRAF3)、干扰素-β(interferon-beta,IFN-β)和 干扰素-λ1(interferon -λ1,IFN-λ1)分子的表达特征,探索胚胎期Toll样受体4(toll like receptor 4,TLR4)-TRIF非依赖性信号通路与肠管再通过程的相关性。 方法 采用免疫组化技术观察和比较新生儿正常肠壁和Ⅰ型肠闭锁隔膜组织黏膜层内TRIF通路内重要分子的表达特征。收集16例患儿组织标本(解放军总医院儿科医学部1例,首都儿科研究所1例,河北医科大学第二医院6例,哈尔滨市儿童医院5例,长春市儿童医院3例),均为术中证实的Ⅰ型肠闭锁隔膜组织,为隔膜组;以同一患儿术中行肠切除、肠吻合过程中钳取收集的正常肠壁组织为对照组。患儿手术年龄为出生后1~3 d,其中空肠闭锁13例(男5例,女8例),回肠闭锁3例(男1例,女2例)。将组织标本转入快速组织处理系统进行组织标本连续切片。采用常规HE染色进行定位,隔膜组和对照组各16例,每个病例染色后随机取4个视野(非肌肉区域),应用图像分析软件Image pro plus 6.0测量积分光密度(integral-optical density ,IOD)和面积(Area),并计算出平均吸光度(A)(OD)。OD=IOD/Area。采用t检验和双盲法分析免疫组化平均光密度。 结果 新生儿正常肠壁组织黏膜层的TRIF、TRAF3、IFN-β和IFN-λ1的分子表达不明显或低表达,在隔膜组织黏膜层表达显著。半定量分析结果显示,TLR4、MyD88、TRAF6和IRAK4分子在隔膜组织黏膜层表达比新生儿正常肠壁黏膜层多,二者差异有统计学意义(隔膜组织分别为0.6 316±0.0 268,0.5 066±0.0 378,0.6 091±0.0 270,0.3 841±0.0 610,正常肠壁分别为0.4 333±0.0 409,0.3 758±0.0 365,0.4 108±0.0 263,0.2 108±0.0 573,P值分别为0.0 042,0.0 360,0.0 057,0.0 137。 结论 TLR4/MyD88信号通路参与了Ⅰ型肠闭锁隔膜组织的形成或发展。
Abstract:
Objective To observe the expressions of TRIF,TRAF3,IFN-β and IFN-λ1 in septum derived from type I intestinal atresia and examine their correlations.Methods Immunohistochemistry was utilized for observing and comparing the expressions of important molecules in TRIF pathway in mucosa of normal intestinal wall and type I intestinal atresia diaphragm.A total of 16 tissue samples were collected from Department of Pediatric Medicine,General Hospital of PLA(n=1),Capital Institute of Pediatrics(n=1),Second Hospital,Hebei Medical University(n=6),Harbin Children’s Hospital(n=5)and Changchun Children’s Hospital(n=3).All samples were intraoperatively confirmed as type I intestinal atresia diaphragm tissue(diaphragm group).Normal intestinal wall tissues collected during intestinal resection and anastomosis in the same child were selected as control group.Totally 16 septa in operated neonates at post-birth 1~3 days were collected,including 13 septa(male 5,female 8)derived from jejunum and 3 septa(male 1,female 2)from ileum.Normal intestinal wall tissues were collected as control during surgery in the same neonate.All fresh tissues were processed for serial tissue sectioning and hematoxylin-eosin(HE)& immunohistochemical stains of TRIF,TRAF3,IFN-β and IFN-λ1 molecules in each group for histological observations.Four visual fields under immunohistochemical stain were targeted randomly for semi-quantitative calculation of average optical density(OD)by Image pro plus6.0 software.Statistical methods of t test and double blinding were employed for analyzing the average optical density of immunohistochemistry.Results Immunohistochemical stain indicated that the expressions of TRIF,TRAF3,IFN-β and IFN-λ1 in septum tissue were significant compared with those in normal neonatal intestinal wall.Semi-quantitative calculation indicated marked elevations in septum tissue in contrast to those in normal neonatal intestinal wall(fraction of diaphragm tissue was 0.6 316±0.0 268,0.5 066±0.0 378,0.6 091±0.0 270,0.3 841±0.0 610;normal intestinal wall 0.4 333±0.0 409,0.3 758±0.0 365,0.4 108±0.0 263,0.2 108±0.0 573,p value point ratio 0.0042,0.0 360,0.0 057,0.0 137).Conclusion TLR4/TRIF signaling pathway is involved in formation and development of septum tissue in type I intestinal atresia during fetal period.

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备注/Memo

收稿日期:2021-8-31。
基金项目:北京市自然科学基金(7222015)
通讯作者:刘雪来,Email:liuxuelai_steven@163.com

更新日期/Last Update: 1900-01-01