Sun Rong,Wu Zhouguang,Ye Yongqin,et al.Preliminary study on the expression level and clinical significance of GPC1 in liver tissues of children with biliary atresia[J].Journal of Clinical Pediatric Surgery,2019,18(03):211-215.[doi:10.3969/j.issn.1671-6353.2019.03.010]
GPC1在胆道闭锁患儿肝组织中表达水平及临床意义的初步探索
- Title:
- Preliminary study on the expression level and clinical significance of GPC1 in liver tissues of children with biliary atresia
- Keywords:
- GPC1; Biliary Atresia; Liver/Anatomy &; Histology; Gene Expression Regulation
- 分类号:
- R729;R657.4+4;R393
- 摘要:
- 目的 初步探讨GPC1在胆道闭锁患儿发病过程中的临床意义。方法 选取深圳市儿童医院2016年7月至2018年2月收治的胆道闭锁患儿35例作为观察组，同期选取本院收治的先天性胆总管扩张症患儿42例作为对照组。通过免疫组化结果了解GPC1在胆道闭锁肝脏中的分布情况；通过qPCR及Western Blot对胆道闭锁（BA）及先天性胆总管扩张症（CC）患儿肝脏中的GPC1蛋白水平、GPC1 mRNA水平进行检测。结果 本研究结果显示GPC1主要分布在胆道闭锁肝脏胆管细胞顶部，观察组和对照组患儿GPC1 mRNA表达水平（0.65±0.05 vs. 2.54±0.12，P<0.05）、蛋白表达量（1.31±0.14 vs. 2.83±0.25，P<0.05）比较，差异有统计学意义。结论 GPC1 mRNA和蛋白产物的低表达可能与胆道闭锁的发病密切相关。
- Abstract:
- Objective To explore the clinical significance of GPC1 in the pathogenesis of children with biliary atresia (BA).Methods A total of 35 hospitalized BA children at Children’s Hospital of Shenzhen from July 2016 to February 2018 were recruited into observation group.And another 42 hospitalized children with congenital choledochal dilatation were selected as control group.The distribution of GPC1 protein in observation group was detected by immunohistochemistry and the expression levels of GPC1 and protein expression in liver samples of two groups were detected by quantitative polymerase chain reaction (qPCR) and Western blot.Results GPC1 was distributed predominantly at the top of cholangiocarcinoma cells.The expression of GPC1 mRNA in observation and control groups (0.65±0.05 vs. 2.54±0.12,P<0.05) and protein expression (1.31±0.14 vs. 2.83±0.25,P<0.05) showed statistical differences.Conclusion The expression of GPC1 may be closely correlated with the pathogenesis of BA.
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备注/Memo
收稿日期:2018-09-13。
基金项目:国家自然科学基金（编号：81770512）
通讯作者:王斌,Email:szwb1967@126.com