[1]童玲,臧婧羽,袁小建,等.ＲｈｏＡ／ ＲＯＣＫ 信号通路在先天性巨结肠病变肠段的变化[J].临床小儿外科杂志,2018,17(03):226-230.
　Tong Ling,Zang Jingyu,Yuan Xiaojian,et al.Variation of RhoA/ROCK signaling pathway in colon of Hirschsprungs disease.[J].Journal of Clinical Pediatric Surgery,2018,17(03):226-230.
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ＲｈｏＡ／ ＲＯＣＫ 信号通路在先天性巨结肠病变肠段的变化

《临床小儿外科杂志》[ISSN:1671-6353/CN:43-1380/R] 卷: 第17卷 期数: 2018年03期 页码: 226-230 栏目: 实验研究 出版日期: 2018-03-28
Title:
Variation of RhoA/ROCK signaling pathway in colon of Hirschsprungs disease.
作者:
童玲; 臧婧羽; 袁小建; 等
上海交通大学医学院基础医学院解剖与生理学系
Author(s):
Tong Ling1; 2; 3; Zang Jingyu2; Yuan Xiaojian3; Hong Zhihua3; Wu Wenjie2; 3 Chen Jie2; 3; Xu Wenxie1; 2.
1.Department of Anatomy & Physiology，Shanghai Jiaotong University School of Medicine，Shanghai 200240，China；
关键词:
Ｈｉｒｓｃｈｓｐｒｕｎｇ 病;  Ｒｈｏ 激酶;  肌细胞;  平滑肌;  收缩;  结肠
Keywords:
Hirschsprung Disease;  Rho Kinase Signaling Pathway;  Myocytes;  Smooth Muscle;  Constriction;  Colon
文献标志码:
A
摘要:
目的　观察在先天性巨结肠（ Ｈｉｒｓｃｈｓｐｒｕｎｇｓ ｄｉｓｅａｓｅ，ＨＤ） 病变肠段中，与ＲｈｏＡ／ ＲＯＣＫ 信
号通路相关的蛋白的表达情况，为ＨＤ 患者结肠动力障碍提供新的研究角度。　方法　采用Ｗｅｓｔｅｒｎ
ｂｌｏｔｔｉｎｇ 的方法，分别对９ 例ＨＤ 患儿手术切除的病变段和扩张段肠壁中与ＲｈｏＡ／ Ｒｈｏ 激酶（ ＲｈｏＫ，
ＲＯＣＫ）信号通路相关蛋白的表达进行检测。　结果　９ 例患者狭窄段Ｐ（ Ｓｅｒ１９） ＭＬＣ２０ 的表达水平与扩
张段相比明显减少，差异有统计学意义（ ｔ ＝ ２． ５２７，Ｐ ＝ ０． ０３５） ；在与ＲｈｏＡ／ ＲＯＣＫ 信号通路相关的蛋白
表达中，狭窄段ＲＯＣＫ１ 蛋白表达与扩张段相比明显减少（ ｔ ＝ ３． １０４，Ｐ ＝ ０． ０１５） ，狭窄段ＲＯＣＫ２ 蛋白表
达与扩张段相比减少（ ｔ ＝２． ３５７，Ｐ ＝０． ０４６） ，狭窄段Ｐ（ Ｔｈｒ８５３） ＭＹＰＴ１ 蛋白表达与扩张段相比也减少
（ ｔ ＝２． ８５７，Ｐ ＝０． ０２８） ，差异均有统计学意义。　结论　ＨＤ 患者病变部位平滑肌细胞的ＲｈｏＡ／ ＲＯＣＫ
信号通路明显减弱，说明该途径可能与ＨＤ 的发病过程相关。
Abstract:
ObjectiveTo explore the protein expression of RhoA/ROCK signaling pathway in Hirschsprungs disease (HD) and provide new perspectives for studying colonic dysfunction in HD patients.MethodsThe protein expression involved in RhoA/ROCK signaling pathway was examined by Western blot in 9 HD cases aged from 6 months to 2 years.And statistical software was used for analyzing the results.ResultsThe expression of P(Ser19)MLC20 obviously decreased in narrow segment as compared with dilated counterpart (t=2.527，P=0.035)；and regarding the protein expressions involved in RhoA/ROCK signaling pathway，the expression of ROCK1 obviously decreased in narrow segment as compared with dilated counterpart (t=3.104，P=0.015).The expression of ROCK2 decreased in narrow segment compared with dilated counterpart (t=2.357，P=0.046).The expression of P(Thr853)MYPT1 also decreased in narrow segment as compared with dilated counterpart (t=2.857，P=0.028).And both had statistical significance.ConclusionRhoA/ROCK signaling pathway is down-regulated in HD.And these results may be related to colonic dysfunction of HD patients.
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