[1]刘德鸿、,沈丽萍,蔡蕾,等.睾丸间质细胞特异性表达Cre重组酶转基因小鼠的建立[J].临床小儿外科杂志,2013,12(03):201-204.[doi:10.3969/j.issn.1671—6353.2013.03.011]
　LIU De hong,SHEN Li ping,CAI Lei,et al.Establishment of testicle leydig cells specific Cre transgenic mice[J].Journal of Clinical Pediatric Surgery,2013,12(03):201-204.[doi:10.3969/j.issn.1671—6353.2013.03.011]
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睾丸间质细胞特异性表达Cre重组酶转基因小鼠的建立

《临床小儿外科杂志》[ISSN:1671-6353/CN:43-1380/R] 卷: 第12卷 期数: 2013年03期 页码: 201-204 栏目: 论著 出版日期: 2013-06-28
Title:
Establishment of testicle leydig cells specific Cre transgenic mice
作者:
刘德鸿1、3; 沈丽萍1; 蔡蕾2; 王津津2; 匡颖2; 孙宁3
作者单位：1，上海交通大学医学院附属瑞金医院（上海市，200025）；2，上海南方模式生物研究中心（上海市，200127）；3，首都医科大学附属北京儿童医院（北京市，100045）；通讯作者：孙宁，Email：snsunning@tom.com ；沈丽萍，Email：slp.1305@yahoo.com.cn
Author(s):
LIU Dehong1; 3;  SHEN Liping1;  CAI Lei3;  et al.
1，Ruijin Hospital Shanghai Jiaotong University， Medicine of School， Shanghai 200025，China；2，Biomodel Organism Center of Shanghai， Shanghai 200127，China；3，Beijing children’s Hospital，Capital Medical University， Beijing 100045，China.
关键词:
睾丸;  间质细胞;  动物实验;  基因表达
Keywords:
Ｔｅｓｔｉｓ;  Ｓｔｒｏｍａｉ　Ｃｅｌｌｓ; 　 Ａｎｉｍａｉ　Ｅｘｐｅｒｉｍｅｎｔａｔｉｏｎ;  Ｇｅｎｅ　Ｅｘｐｒｅｓｓｉｏｎ
DOI:
10.3969/j.issn.1671—6353.2013.03.011
摘要:
目的苗勒氏管激素受体—2（antimullerian hormone receptor—2，Amhr2）是睾丸间质细胞中的特异性标志分子，我们构建了Amhr2基因启动子介导的睾丸间质细胞特异性表达Cre重组酶转基因小鼠(Amhr2—Cre)。方法采用显微注射法将7.1 kb的转基因片段导入小鼠基因组，获得子代小鼠睾丸组织进行PCR检测，再将转基因小鼠与ROSA26报告小鼠交配，利用LacZ染色对Amhr2—Cre双转基因进行检测。结果获得子代Amhr2Cre转基因小鼠经PCR检测显示，睾丸间质细胞中Cre重组酶介导的Amhr2基因发生重组；LacZ染色进一步表明，Cre重组酶在睾丸间质细胞中特异性表达，并介导ROSA位点LoxP序列间的重组。结论建立的睾丸间质细胞特异性表达Cre重组酶的转基因小鼠在睾丸组织中具有一定的组织特异性，并能在体内成功介导这些睾丸间质细胞基因组上LoxP位点间的重组，是一种研制在睾丸特定细胞中特异性基因剔除小鼠的良好遗传工具小鼠。
Abstract:
Objetive Amhr2 protein is the specific protein of testicle tissue. We generated a new transgenic line expressing Cre recombinase under the control of an Amhr2 gene promoter (Amhr2Cre) to investigate sexual development.MethodsMicroinjection was employed to introduce the 7.1 kb transgenic fragment into oocytes，and progenies were obtained. PCR detected expression of Amhr2Cre transgene within testicle tissue containing leydig cells. Transgenic line was crossed with ROSA26 report line， the activity of Cre recombinase was revealed by LacZ staining in ROSA26; Amhr2Cre double transgenic mice.ResultsPCR products revealed that the Amhr2 gene was deleted by Cre mediated recombination in the leydig cells. LacZ staining of the Amhr2Cre and ROSA26 double transgenic mice showed that Cre recombinase activity was detected in leydig cells.ConclusionAll these data indicated that the Cre recombinase has been expressed in the testicle tissues of the Amhr2Cre transgenic mice．The mice generated could serve as a useful tool for generating testicle specific geneknockout mice.

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备注/Memo

基金项目：国家自然科学基金项目(编号：30700830)

更新日期/Last Update: 2013-06-30