[1]邢莉莉,吴晔明,张弛.熊果酸对神经母细胞瘤细胞增殖凋亡及 MYCN表达的影响[J].临床小儿外科杂志,2013,12(03):196-200.[doi:10.3969/j.issn.1671—6353.2013.03.010]
　XING Li li,WU Ye ming,ZHANG Chi..Ursolic acid effects proliferation、apoptosis and MYCN expression of neuroblastoma cell[J].Journal of Clinical Pediatric Surgery,2013,12(03):196-200.[doi:10.3969/j.issn.1671—6353.2013.03.010]
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熊果酸对神经母细胞瘤细胞增殖凋亡及 MYCN表达的影响

《临床小儿外科杂志》[ISSN:1671-6353/CN:43-1380/R] 卷: 第12卷 期数: 2013年03期 页码: 196-200 栏目: 论著 出版日期: 2013-06-28
Title:
Ursolic acid effects proliferation、apoptosis and MYCN expression of neuroblastoma cell
作者:
邢莉莉; 吴晔明; 张弛
作者单位：上海交通大学医学院附属新华医院(上海市，200092)，通讯作者：张弛，Email：zhangchi31@126.com
Author(s):
XING Lili;  WU Yeming;  ZHANG Chi.
Department of Pediatric Surgery, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.
关键词:
神经母细胞瘤;  熊果酸;  细胞增殖;  细胞凋亡;  MYCN
Keywords:
Neuroblastoma;  Ursolic acid;  Cell proliferation;  Cell apoptosis;  MYCN
DOI:
10.3969/j.issn.1671—6353.2013.03.010
摘要:
目的实验研究熊果酸作用于体外培养的神经母细胞瘤细胞株(SH—SY—5Y)，对肿瘤细胞增殖、凋亡及MYCN基因mRNA表达的影响，探讨熊果酸对儿童神经母细胞瘤的治疗的作用。方法以终浓度为4uM/L、8uM/L、12uM/L的熊果酸作用于神经母细胞瘤细胞SH—SY—5Y，WST—1法检测SH—SY—5Y细胞增殖情况，流式细胞术检测SH—SY—5Y细胞凋亡情况，采用Real time PCR方法检测MYCN mRNA的表达情况。结果熊果酸对SH—SY—5Y细胞增殖的抑制呈明显的剂量、时间依赖性，随剂量的增加，作用时间的延长，抑制效果逐渐加强；熊果酸对SHSY—5Y细胞凋亡的促进呈明显的剂量依赖性，随剂量的增加，促进凋亡效果逐渐加强；熊果酸对SH—SY—5Y细胞MYCN表达有抑制作用并有明显的剂量依赖性，随剂量的增加，抑制效果逐渐加强。12uM/L作用72 h后神经母细胞瘤细胞增殖抑制效率达98.3%，MYCN基因mRNA表达抑制率达52.37％。结论应用熊果酸在体外环境作用SH—SY—5Y细胞，可以有效抑制该细胞的增殖、促进细胞凋亡、抑制原癌基因MYCN mRNA的表达。
Abstract:
Objetive This study was designed to investigate the effects of ursolic acid in proliferation、apoptosis and MYCN gene mRNA expression of SHSY5Y cell line cultured in vitro, in order to investigate the effect of ursolic acid (UA) in neuroblastoma(NB).MethodNeuroblastoma SHSY5Y cells were treated by 4 uM/L、8 uM/L、12 uM/L ursolic acid.WST1 was used to measure the levels of the proliferation of SHSY5Ycells cultured with different concentrations of ursolic acid. While SHSY5Ycells apoptosis was measured by flow cytometry. MYCN gene mRNA expression in SHSY5Yceils was measured using real time PCR.ResultsThe proliferation of SHSY5Ycells was obviously inhibited by ursolic acid in a doseand timedependent manner. The apoptosis of SHSY5Ycells was obviously promoted by ursolic acid in a dosedependent manner. The MYCN gene mRNA expression in SHSY5Ycells was obviously inhibited by ursolic acid in a doseependent manner. Ursolic acid of 12uM/L treatment for 72 hrs produced a best effect with an inhibitory rate of SHSY5Y cell proliferation of 98.3 % and inhibitory rate of MYCN gene mRNA expression of 52.37%. ConclusionsUrsolic acid may inhibit the growth of neuroblastoma cells, promote neuroblastoma cells apoptosis and downregulate MYCN mRNA expression.

参考文献/References:

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更新日期/Last Update: 2013-06-30