ZHANG Yuan,WANG Chang-lin.Expression of melanoma antigen-1 mRNA in nephroblastoma and its clinical significance[J].Journal of Clinical Pediatric Surgery,2013,12(02):90-92.[doi:10.3969/j.issn.1671— 6353.2013.02.004]
肾母细胞瘤黑色素抗原基因 — 1 mRNA的表达及临床意义
- Title:
- Expression of melanoma antigen-1 mRNA in nephroblastoma and its clinical significance
- 关键词:
- 基因; 肾母细胞瘤; RNA指导的DNA聚合酶
- Keywords:
- Genes; Wilms Tumor; RNA-Directed DNA Polymerase
- 摘要:
- 目的 探讨小儿肾母细胞瘤中黑色素抗原基因-1(MAGE-1)mRNA的表达及意义。 方法 用逆转录多聚酶链反应(RT-PCR)方法检测32例肾母细胞瘤组织、10例瘤旁组织、6例无瘤肾组织的MAGE-1 mRNA的表达情况,并分析MAGE-1 mRNA的表达与肾母细胞瘤的病理分型、临床分期之间的关系。 结果 所有标本均有不同程度的MAGE-1 mRNA表达,表达程度按肿瘤组织、瘤旁组织、无瘤组织依次降低。肿瘤组织是瘤旁组织的1.40倍(t=0.853,P>0.05),是无瘤对照组的2.58倍(t =2.35,P<0.05)。MAGE-1 mRNA在肾母细胞瘤组织中的表达与病理分型及临床分期均无关(P>0.05)。 结论 MAGE-1 mRNA在肾母细胞瘤组织中呈高表达,提示MAGE-1 mRNA可望成为肾母细胞瘤免疫治疗的靶抗原。
- Abstract:
- Objetive To investigate the mRNA expression of melanoma antigen-1 mRNA in Nephroblastoma and its’clinical significance. Methods MAGE-1 mRNA expression were determined by RT-PCR in 32 cases of nephroblastoma tissues and 10 cases of paracancer renal tissues and 6 cases of renal tissue without tumour.The relations about MAGE-1 mRNA expression were also analyzed in nephroblastoma’s different clinical stage and different pathology type. Results Some degree of MAGE-1 mRNA expression was present in all samples.The MAGE-1 mRNA level was 1.4-fold higher in tumour tissues than in paracancer renal tissues(t=0.853,P>0.05)and 2.58-fold higher than in renal tissue without tumour(t=2.35,P<0.05).We also enduce that MAGE-1 mRNA level do not correlate with pathology type and clinical stage in nephroblastoma(P>0.05). Conclusion MAGE-1 mRNA in nephroblastoma is expressed so high that it has important significance for immunotherapy and prognosis of nephroblastoma.
参考文献/References:
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