金雀异黄素对血管增生性视网膜病变小鼠中VEGF表达的影响
- 文献标志码:
- A
- 摘要:
- 目的研究相对缺氧对小鼠视网膜血管内皮生长因子( vascular endothelial growth factor, VEGF) 表达的诱导作用，以及金雀异黄素( genistein, Gen) 对其表达的影响和机理。方法制作缺氧小鼠模型，采用视网膜切片计数视网膜血管的增生，免疫荧光法检测视网膜中VEGF的表达，分析不同Gen浓度（0、50、100、200mg/kg）对缺氧致血管增生性视网膜病变小鼠的VEGF表达的影响。结果正常对照组小鼠视网膜切面有少量血管内皮细胞核，0剂量组（相对缺氧组）视网膜切片中突破内界膜的血管内皮细胞核数较对照组显著增加（P<0.001），提示相对缺氧诱导视网膜血管新生，表明动物模型制作成功。给药组突破内界膜的血管内皮细胞核数均较相对缺氧组减少，并且呈剂量依赖性，提示Gen可抑制缺氧诱导的视网膜血管新生。与对照组相比，相对缺氧组小鼠视网膜内层VEGF的表达明显增强；Gen给药组VEGF的表达较相对缺氧组减弱。结论Gen可抑制缺氧诱导的VEGF的表达，提示Gen 对视网膜新生血管的形成具有预防和潜在的临床价值。
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