DONG Chun-qiang,YANG Ti-quan,DONG Kun.Expression of hypoxia inducible factor-1 alpha in pediatric nephroblastoma[J].Journal of Clinical Pediatric Surgery,2013,12(02):85-89.[doi:10.3969/j.issn.1671— 6353.2013.02.003]
缺氧诱导因子-1α在小儿肾母细胞瘤中的表达
- Title:
- Expression of hypoxia inducible factor-1 alpha in pediatric nephroblastoma
- 摘要:
- 目的 研究缺氧诱导因子-1α(hypoxia-inducible factor-1α,HIF-1α)在小儿肾母细胞瘤(wilms tumor,WT)中的表达及其与临床病理学参数、微血管密度(microvessel density,MVD)和预后的关系。 方法 选取本院2008年1月至2012年1月经手术切除的WT肿瘤标本45例,分别根据年龄、性别、病理分型、临床分期、NWTS-5高低危因素进行分组,采用免疫组织化学方法测定WT组织和瘤旁组织中HIF-1α和CD 34的表达,通过CD 34标记血管内皮细胞并计算MVD,结合临床资料和随访结果对实验数据进行统计学分析。 结果 WT组HIF-1α的阳性率高于瘤旁组(95.6% vs 40%,P<0.05),HIF-1α在WT 中的表达与与临床分期相关,P<0.05; WT组MVD高于瘤旁组(58.22±10.009 vs(49.43±8.114),P<0.05,HIF-1α的表达与MVD的相关系数为r = 0.402,P<0.05; HIF-1α表达阳性程度高危组高于低危组,P<0.05,HIF-1α低表达组术后累计生存率显著高于高表达组,P<0.05。 结论 HIF-1α的高表达与WT的生长有关,可促进肿瘤新生血管的生成,并提示小儿WT的预后不良。
- Abstract:
- Objetive The aim of this study was to explore the expression of hypoxia-inducible factor-1α(HIF-1α)in children with wilms tumor(WT)and its correlation with clinicopathology, microvessel density(MVD)and prognosis. Methods 45 cases with nephroblastoma resecting,which specimens were confirmed after surgical pathologically in our unit from January 2008 to January 2012,and they were divided into five groups by age, sex, histopathological type, clinical stage and NWTS-5 risk factors, respectively. The expression of HIF-1α and CD 34 in WT organizations and adjacent tissues was tested by immunohistochemical method and MVD was calculated by CD 34 staining,which were analysis combined with the clinical data and results of the follow-up. Results The ratio of HIF-1α expressed in WT group was higher than adjacent tissues group(95.6% vs 40%,P<0.05)and intensity was increased with the increasing of clinic stages of the tumor(P<0.05). MVD was higher in WT group than in adjacent tissue groups(58.22±10.009 vs 49.43±8.114,P<0.05)and had a positive correlation to HIF-1α(P<0.05). HIF-1α expressed in high risk group was higher than in low risk group(P<0.05)and the postoperative cumulated survival rate was higher in low expression of HIF-1α group than in the high expression group(P<0.05). Conclusions High expression of HIF-1α is correlated to the development of WT and improves angiogenesis, which indicates a poor prognosis.
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