CHEN Ren wei,ZHU Xin yuan,ZHOU Wen wu,et al.Protective effects of ulinastatin on cellular immune function in infants underwent cardiac operations with cardiopulmonary bypass.[J].Journal of Clinical Pediatric Surgery,2011,10(01):0.
乌司他丁对婴幼儿体外循环细胞免疫功能的保护作用
- Title:
- Protective effects of ulinastatin on cellular immune function in infants underwent cardiac operations with cardiopulmonary bypass.
- Keywords:
- Extracorporeal Circulation; Lymphocytes/IM; Infant
- 摘要:
- 目的观察体外循环对婴幼儿T淋巴细胞免疫功能的影响，及乌司他丁对免疫功能的保护作用。方法将湖南省儿童医院2010年1月至2010年6月收治的连续50例体重10kg以下的室间隔缺损患儿随机分为两组，乌司他丁组和（U组）对照组（C组），每组25例。U组在体外循环预充液中加入乌司他丁10000U/kg，C组未使用乌司他丁。选取CPB启动前、结束后1h、1d、7d共4个时间点，采集静脉血以流式细胞仪行T淋巴细胞亚群分析。结果术后1d U组CD4+高于C组(P<0.05)，CD3+及CD4+/CD8+明显高于C组(P<0.01)，CD8+明显低于C组(P<0.01)。术后7d U组CD3+、CD4+、CD4+/CD8+明显高于C组(P<0.01)。结论体外循环可抑制婴幼儿T淋巴细胞免疫功能；乌司他丁能够改善体外循环造成的免疫抑制。
- Abstract:
- ObjectiveTo observe the change of T lymphocyte immunity functions after cardiopulmonary bypass (CPB) in infants, and to evaluate the protective effects of ulinastatin on cellular immune function. MethodsFrom Jan. 2010 to Jun. 2010, there were 50 infants with ventricular septal defects, whom divided randomly into the Ulinastatin group or Control, enrolled into this prospective trial. And infants from the ulinastatin group were treated with 1000 U/kg which was added into the CPB priming fluid during the correct operations. Blood samples were taken at different time points for the analysis of T lymphocyte and its subtype by flow cytometry.Results24 hours after the correct operations, and compared with the control group, infants from the ulinastatin group had higher levels of CD4+, CD3+ and CD4+/CD8+, as well as a lower level of CD8+. And 7days late, the levels of CD4+, CD3+ and CD4+/CD8+ in the ulinastatin group were still higher than those in the control group.ConclusionsAdministration of ulinastatin in the CPB priming fluid can attenuate the inhibition of T lymphocyte immunity functions caused by CPB in infants.
参考文献/References:
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4郭巍,邹承伟,王安彪,等.西咪替丁对体外循环手术患者免疫功能的保护作用\[J\].中国胸心血管外科临床杂志, 2007, 14(2): 97—100.
5雷宾峰,陈铭伍,冼磊,等.乌司他丁对心脏不停跳心内直视术机体免疫功能的影响\[J\].广西医科大学学报, 2008, 25(3):381—383.
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备注/Memo
作者单位：湖南省儿童医院心胸外科（长沙市，410007），本课题受湖南省自然科学基金资助（项目号06TJ41078)