Hu He,Jiang Fei,Ji Guangchun,et al.Effect of light repair on spinal cord tether with neurogenic bladder in rats[J].Journal of Clinical Pediatric Surgery,2020,19(07):648-652.[doi:10.3969/j.issn.1671-6353.2020.07.017]
光修复对脊髓栓系伴神经源性膀胱大鼠的作用研究
- Title:
- Effect of light repair on spinal cord tether with neurogenic bladder in rats
- Keywords:
- Neural Tube Defects; Urinary Bladder; Neurogenic; Light Gene; Nerve Growth Factor; Child
- 分类号:
- R726.9;R694+.5;R628
- 摘要:
- 目的 初步评估光感基因技术治疗脊髓栓系伴神经源性膀胱未成年大鼠模型的疗效。方法 将46只未成年雌性大鼠随机分为健康对照组（15只）、脊髓栓系有光感基因组（15只）和脊髓栓系无光感基因组（16只），通过免疫荧光染色观察腺病毒载体Ad-EGFP-ChR2转染情况；经特定蓝光照射2周后进行膀胱尿动力学测定，采用ELISA法和Western blot法分别检测尿液、膀胱组织中NGF的表达情况。结果 与脊髓栓系无光感基因组相比，脊髓栓系有光感基因组膀胱逼尿肌漏点压降低、最大容量增加、顺应性升高、残余尿量减少、尿液中NGF含量减少、NGF/Cr值降低、膀胱组织中NGF蛋白表达量降低，上述指标的变化差异均有统计学意义（P<0.05）。与健康对照组相比，脊髓栓系无光感基因组中以上各项指标差异均无统计学意义（P>0.05）。结论 动物实验结果表明，光感基因技术对恢复脊髓栓系诱发的神经源性膀胱功能有一定的帮助，为儿童脊髓栓系伴神经源性膀胱功能障碍的治疗提供了实验理论基础。
- Abstract:
- Objective To evaluate the efficacy of photoreceptor gene therapy for spinal cord tethering with neurogenic bladder in a juvenile rat model preliminarily.Methods Forty-six female young rats were randomly divided into three groups of healthy control (15 rats),tethered cord with light gene (15 rats) and tethered cord without light gene (16 rats).The juvenile rat model of spinal cord tethered with neurogenic bladder was established.The transfection of adenovirus vector Ad-EGFP-ChR2 was observed by immunofluorescent staining.After 2-week specific blue light irradiation,bladder urodynamics,enzyme-linked immunosorbent assay (ELISA) and Western blot were performed for detecting nerve growth factor (NGF) in urine and bladder tissues.Results .As compared with tethered cord without light gene group,bladder detrusor leakage point pressure and residual urine volume decreased,maximum volume and compliance increased,NGF content in urine dropped,NGF/Cr value declined,the expression of NGF protein in bladder tissue decreased in tethered cord with light gene group. The difference in the above indicators was statistically significant.As compared with healthy control group,no significant difference existed in the above indices of tethered cord without light gene group (P>0.05).Conclusion The results of animal experiments show that photorecepter gene technology may restore neurogenic bladder functions induced by spinal cord thrombosis.It provides experimental therapeutic rationales for children with spinal cord tether with neurogenic bladder dysfunction.
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备注/Memo
收稿日期:2019-05-12。
基金项目:辽宁省科学技术基金（编号：20170540216）
通讯作者:蒋飞,Email:jf2299@163.com